Incorporation of isotopically enriched amino acids.
Incorporation of isotopically enriched amino acids.
复制标题
掺入富含同位素的氨基酸。
DOI：
10.1002/0471140864.ps2603s47
复制
发表时间：
2007
影响因子：
--
通讯作者：
Long,JoannaR
中科院分区：
文献类型：
--
作者：
Samuel-Landtiser,Mini;Zachariah,Cherian;Williams,ChrisR;Edison,ArthurS;Long,JoannaR
将同位素标记掺入蛋白质对于应用核磁共振 (NMR)、X 射线或中子衍射晶体学以及质谱 (MS) 方法来研究蛋白质的结构和动力学非常有用。本单元介绍了通过在大肠杆菌中表达将同位素标记整合到蛋白质中的方法。大肠杆菌和杆状病毒转染Sf9昆虫细胞或通过无细胞方式。该单元还介绍了通过化学手段将同位素标记引入通过固相肽合成的合成肽中或通过不稳定蛋白质基团的化学修饰引入分离的蛋白质中的方法。
The incorporation of isotope labels into proteins is extremely useful for the application of nuclear magnetic resonance (NMR), X‐ray or neutron‐diffraction crystallography, and mass spectrometry (MS) methodologies to investigate the structure and dynamics of proteins. This unit presents methods for incorporating isotopic labels into proteins via expression inE. coliand baculovirus transfectedSf9 insect cells or through cell‐free means. The unit also presents methods for introducing isotopic labels by chemical means into synthetic peptides by solid phase peptide synthesis or into isolated proteins by chemical modification of labile protein groups.