Quantitative high resolution mapping of HvMLH3 foci in barley pachytene nuclei reveals a strong distal bias and weak interference.
Quantitative high resolution mapping of HvMLH3 foci in barley pachytene nuclei reveals a strong distal bias and weak interference.
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大麦粉状核中HVMLH3焦点的定量高分辨率映射显示出强烈的远端偏见和弱干扰。
DOI：
10.1093/jxb/ert079
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发表时间：
2013-05
影响因子：
6.9
通讯作者：
Jenkins G
中科院分区：
文献类型：
--
作者：
Phillips D;Wnetrzak J;Nibau C;Barakate A;Ramsay L;Wright F;Higgins JD;Perry RM;Jenkins G
在大麦（Hordeum vulgare L.）中，交叉位点（遗传杂交的物理位点）向染色体的远端倾斜，有效地将大部分基因委托给重组冷点。这限制了潜在的遗传变异，降低了这种作物基于图谱的克隆和育种方法的效率。从实现物种遗传潜力的角度来看，通过正向和反向遗传手段将重组位点转移到更近端的染色体区域可能是有益的，但这是基于对这些事件位点的控制机制的更好理解，以及对重组模式真正变化的识别能力的前提。分离出大麦MutL同源物（HvMLH3）作为一类干扰交叉的标记物，并提出了一种特异性抗体。HvMLH3和突触复合体横丝蛋白ZYP1的免疫定位，结合特异性大麦染色体的荧光原位杂交（FISH）标记，已经能够进入大麦品种Morex和Bowman的物理重组景观。在整个基因组中，HvMLH3位点的远端定位一致，并且在二价2H和3H中观察到相似的HvMLH3位点模式。这两个品种之间HvMLH3疫源地总数的差异已被量化，这被解释为代表I类交叉频率的基因型差异。由连锁分析得出的HvMLH3焦点频率与交叉频率之间的差异表明，大麦中至少存在两条交叉途径。与其他植物相比，HvMLH3焦点的干扰相对较弱。
In barley (Hordeum vulgare L.), chiasmata (the physical sites of genetic crossovers) are skewed towards the distal ends of chromosomes, effectively consigning a large proportion of genes to recombination coldspots. This has the effect of limiting potential genetic variability, and of reducing the efficiency of map-based cloning and breeding approaches for this crop. Shifting the sites of recombination to more proximal chromosome regions by forward and reverse genetic means may be profitable in terms of realizing the genetic potential of the species, but is predicated upon a better understanding of the mechanisms governing the sites of these events, and upon the ability to recognize real changes in recombination patterns. The barley MutL Homologue (HvMLH3), a marker for class I interfering crossovers, has been isolated and a specific antibody has been raised. Immunolocalization of HvMLH3 along with the synaptonemal complex transverse filament protein ZYP1, used in conjunction with fluorescence in situ hybridization (FISH) tagging of specific barley chromosomes, has enabled access to the physical recombination landscape of the barley cultivars Morex and Bowman. Consistent distal localization of HvMLH3 foci throughout the genome, and similar patterns of HvMLH3 foci within bivalents 2H and 3H have been observed. A difference in total numbers of HvMLH3 foci between these two cultivars has been quantified, which is interpreted as representing genotypic variation in class I crossover frequency. Discrepancies between the frequencies of HvMLH3 foci and crossover frequencies derived from linkage analysis point to the existence of at least two crossover pathways in barley. It is also shown that interference of HvMLH3 foci is relatively weak compared with other plant species.
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影响因子：
3
作者：
Gauthier F;Martin OC;Falque M
通讯作者：
Falque M
影响因子：
2.6
作者：
Corredor, Eduardo;Naranjo, Tomas
通讯作者：
Naranjo, Tomas
影响因子：
9.8
作者：
Broman, KW;Weber, JL
通讯作者：
Weber, JL
DOI：
10.1007/978-1-60761-103-5_21
发表时间：
2009-01-01
期刊：
MEIOSIS, VOL 2: CYTOLOGICAL METHODS
影响因子：
--
作者：
de Boer, Esther;Lhuissier, Franck G. P.;Heyting, Christa
通讯作者：
Heyting, Christa
影响因子：
11.6
作者：
Falque, Matthieu;Anderson, Lorinda K.;Martin, Olivier C.
通讯作者：
Martin, Olivier C.