Molecular adaptation of Borrelia burgdorferi in the murine host.
Molecular adaptation of Borrelia burgdorferi in the murine host.
复制标题
小鼠宿主中伯氏毛毛毛毛毛毛虫的分子适应。
DOI：
10.1084/jem.20020770
复制
发表时间：
2002-07-15
影响因子：
15.3
通讯作者：
Fikrig, Erol
中科院分区：
文献类型：
--
作者：
Liang, Fang Ting;Nelson, F Kenneth;Fikrig, Erol
对小鼠感染过程中137个脂蛋白基因表达的分析揭示了莱姆病螺旋体伯氏疏螺旋体的两步分子适应。第一步，无论最初接种的伯氏疏螺旋体是否表达了137个脂蛋白基因中的全部（培养螺旋体）或少于40个（宿主适应螺旋体），在将螺旋体引入小鼠宿主后10 d内调节螺旋体转录116个基因。这一步的适应是由宿主组织的微环境诱导的。在感染后17 ~ 30 d的第二阶段，受宿主免疫选择压力的影响，伯氏螺旋体下调了大部分脂蛋白基因，137个基因中只有不到40个表达。这种新的适应机制可能是伯氏疏螺旋体进入慢性感染的关键步骤，因为如果螺旋体未能经历这一过程，病原体将在感染早期被清除。
An analysis of expression of 137 lipoprotein genes on the course of murine infection revealed a two-step molecular adaptation by Borrelia burgdorferi, the Lyme disease spirochete. For the first step, regardless whether the initial inocula of B. burgdorferi expressed either all (cultured spirochetes) or less than 40 (host-adapted spirochetes) of the 137 lipoprotein genes, the spirochetes were modulated to transcribe 116 of the genes within 10 d after being introduced to the murine host. This step of adaptation was induced by the microenvironment of the host tissue. During the second step, which was forced by host immune selection pressure and occurred between 17 and 30 d after infection, B. burgdorferi down-regulated most of the lipoprotein genes and expressed less than 40 of the 137 genes. This novel adaptation mechanism could be a critical step for B. burgdorferi to proceed to chronic infection, as the pathogen would be cleared at the early stage of infection if the spirochetes failed to undergo this process.