ASSOCIATION OF GERMFREE MICE WITH BACTERIA ISOLATED FROM NORMAL MICE.
ASSOCIATION OF GERMFREE MICE WITH BACTERIA ISOLATED FROM NORMAL MICE.
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DOI：
10.1084/jem.122.1.77
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发表时间：
1965-07-01
期刊：
影响因子：
--
通讯作者：
COSTELLO R
中科院分区：
文献类型：
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作者：
SCHAEDLER RW;DUBS R;COSTELLO R
给无菌小鼠喂食被从普通健康小鼠中分离出的各种细菌的纯培养物污染的食物。单独或联合或以每周的间隔连续给予培养物。无论采用何种给药技术，乳酸杆菌和厌氧链球菌都会立即在整个胃肠道中扎根，并与器官壁紧密相连。相比之下，拟杆菌群的生物体仅在大肠中大量发现。暴露后一周内，这三种细菌的数量达到了与普通小鼠相似的水平。在整个观察期间（几个月），它们保持在这些特征水平。它们的存在导致盲肠尺寸逐渐减小，最终在总体外观上变得正常。大肠菌在无菌小鼠胃肠道的所有部位广泛繁殖并持续保持高水平，即使这些部位已经被乳杆菌、厌氧链球菌和拟杆菌定植。然而，在给动物喂食健康无病原体小鼠的肠道内容物后几天内，大肠菌群数量急剧下降。
Germfree mice were given food contaminated with pure cultures of various bacterial species isolated from ordinary healthy mice. The cultures were given singly, or in association, or consecutively at weekly intervals. Whatever the technique of administration, the lactobacilli and anaerobic streptococci immediately established themselves throughout the gastrointestinal tract, and became closely associated with the walls of the organs. In contrast, the organisms of the bacteroides group were found in large numbers only in the large intestine. Within a week after exposure, the populations of these three bacterial species reached levels similar to those found in ordinary mice. They remained at these characteristic levels throughout the period of observation (several months). Their presence resulted in a progressive decrease in the size of the cecum which eventually became normal in gross appearance. Coliform bacilli multiplied extensively and persisted at high levels in all parts of the gastrointestinal tract of germfree mice, even after these had become colonized with lactobacilli, anaerobic streptococci and bacteroides. However, the coliform population fell precipitously within a few days after the animals were fed the intestinal contents of healthy pathogen-free mice.