H-3 THYMIDINE UPTAKE BY THE RAT URINARY-BLADDER AFTER INDUCTION OF DIABETES-MELLITUS
H-3 THYMIDINE UPTAKE BY THE RAT URINARY-BLADDER AFTER INDUCTION OF DIABETES-MELLITUS
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DOI：
10.1016/s0022-5347(17)35768-3
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发表时间：
1993-10-01
影响因子：
6.6
通讯作者：
LONGHURST, PA
中科院分区：
文献类型：
--
作者：
EIKA, B;LEVIN, RM;LONGHURST, PA
链脲佐菌素诱导的糖尿病导致多尿、膀胱质量增加和排尿改变。监测用链脲佐菌素诱导糖尿病后排尿和膀胱质量的时间变化，并与DNA合成和H-3-胸苷掺入相关。在诱导糖尿病后1天内，饮水量、尿排泄量、排尿频率、平均和最大排尿量均增加。这些参数在6至11天内达到最大值，并在30和60天保持不变。膀胱质量显著升高7天，并没有随着糖尿病持续时间的增加而进一步增加。糖尿病4、7和14天组膀胱DNA浓度均降低。H-3-胸苷掺入到DNA中增加2天内诱导糖尿病后，在4至7天达到最大值，并下降到控制值的14天。放射自显影显示诱导糖尿病后一天强烈标记的尿路上皮，标记保持高至第7天。结缔组织和平滑肌标记发育较慢。平滑肌的标记是短暂的，仅在第4天和第7天出现。事件的时间进程与膀胱扩张或排尿量增加刺激胸苷掺入DNA，导致膀胱质量增加的假设一致。
Streptozotocin-induced diabetes mellitus causes diuresis, increases in bladder mass and changes in micturition. Temporal changes in micturition and bladder mass after induction of diabetes with streptozotocin were monitored and correlated with DNA synthesis and H-3-thymidine incorporation. There were increases in water consumption, urine excretion, urinary frequency, and mean and maximal micturition volume within 1 day after induction of diabetes. These parameters reached maximal values within 6 to 11 days and were maintained at 30 and 60 days. Bladder mass was significantly elevated by 7 days and did not increase further with increasing duration of diabetes. DNA concentration was decreased in bladders from 4, 7 and 14 day diabetics. H-3-thymidine incorporation into DNA increased within 2 days after induction of diabetes, reached maximal values at 4 to 7 days and declined to control values by 14 days. Autoradiography showed intense labelling of the urothelium one day after induction of diabetes, with labelling remaining high up to day 7. Connective tissue and smooth muscle labelling were slower to develop. Labelling of smooth muscle was transient, appearing only on days 4 and 7. The time course of the events was consistent with the hypothesis that bladder distension or increasing micturition volume stimulate thymidine incorporation into DNA, resulting in an increase in bladder mass.