Fate of Carbon-14-Labeled Cell Walls in Silage Fermentation
Fate of Carbon-14-Labeled Cell Walls in Silage Fermentation
复制标题
青贮饲料发酵中碳 14 标记细胞壁的命运
DOI：
10.2134/agronj1970.00021962006200040032x
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发表时间：
1970
期刊：
影响因子：
--
通讯作者：
C. H. Gordon
中科院分区：
文献类型：
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作者：
H. Goering;L. Smith;S. Laksmanan;C. H. Gordon
将均匀碳-14标记的小麦植株细胞壁(CW-UL？⁴C)与磨碎的鸭茅干草基质结合并青贮，以确定细胞壁对青贮发酵最终产物的贡献。在半品脱瓶中的8个样品被发酵49、76、141、188、237、285、429或504小时。碳-14的活度是在水溶性、有机酸和二氧化碳中测量的。在乳酸和醋酸中发现了大量的活性，这两种物质最初的增长速度基本相同。8%的标记细胞壁被水解，显然是通过细菌机制。80%存在于酸中，4.5%存在于二氧化碳中。这项技术在研究青贮发酵的各种分支方面是有用的。
Uniformly carbon-14 labeled wheat plant cell walls (CW-UL¹⁴C) were combined with ground orchardgrass hay substrate and ensiled in order to determine the contribution of cell walls to silage fermentation end-products. Eight samples in half-pint jars were fermented for either 49, 76, 141, 188, 237, 285, 429, or 504 hours. Carbon-14 activity was measured in water solubles, organic acids, and carbon dioxide. Substantial activity was found in lactic and acetic acid, both of which increased at essentially the same rate initially. Eight percent of the labeled cell wall was hydrolyzed, apparently through bacterial mechanisms. Eighty percent was found in the acids and 4.5% in carbon dioxide. The usefulness of this technique for studying various ramifications of silage fermentation is demonstrated.