cDNA cloning of a renal Na(+)-Ca2+ exchanger.
cDNA cloning of a renal Na(+)-Ca2+ exchanger.
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肾 Na()-Ca2 交换器的 cDNA 克隆。
DOI：
10.1152/ajprenal.1992.262.6.f1105
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
Shugrue,CA
中科院分区：
文献类型：
--
作者：
Reilly,RF;Shugrue,CA
在本研究中，利用聚合酶链式反应和文库筛选的方法，在与犬心脏肌膜序列(D.A.Nicoll，S.Longoni和K.D.Philipson)同源性的基础上，克隆了兔肾脏Na(+)-Ca~(2+)交换器的cDNA.科学洗手间。DC 250：562-565,1990)。这两个序列之间有很高的相似性，在疏水膜相关结构域、细胞质结构域和3‘-非翻译区的核苷酸同源性分别为95%、89%和90%。兔肾基因编码941个氨基酸，比犬肾短29个氨基酸，相对分子质量为105,121。推导的氨基酸序列在膜相关区域有96%的同源性，在细胞质区域有94%的同源性。Northern印迹分析表明，该基因在肾皮质中表达。在延髓中未检测到任何表达。这一结果与显微穿刺术的研究结果一致，该研究表明Na(+)-Ca~(2+)交换器在皮质而不是髓质肾单位节段具有活性。
In the present study, the polymerase chain reaction (PCR) and library screening were used to clone a cDNA for a rabbit kidney Na(+)-Ca2+ exchanger on the basis of homology with the canine cardiac sarcolemmal sequence (D. A. Nicoll, S. Longoni, and K. D. Philipson. Science Wash. DC 250:562-565, 1990). There is a high degree of similarity between the two sequences, with nucleotide identities of 95, 89, and 90% in the hydrophobic membrane-associated domain, cytoplasmic domain, and 3'-untranslated region, respectively. The rabbit kidney cDNA encodes a predicted protein of 941 amino acids, 29 amino acids shorter than the canine sequence, with a relative molecular weight of 105,121. The deduced amino acid sequence is 96% identical in the membrane-associated domain and 94% identical in the cytoplasmic domain. Northern blot analysis reveals that the cDNA is expressed in the renal cortex. No expression is detected in the medulla. This result is in agreement with micropuncture studies that show Na(+)-Ca2+ exchanger activity in cortical but not medullary nephron segments.