In Pursuit of Zero: Polymer Brushes that Resist the Adsorption of Proteins
In Pursuit of Zero: Polymer Brushes that Resist the Adsorption of Proteins
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DOI：
10.1002/adma.200900383
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发表时间：
2009-06-19
影响因子：
29.4
通讯作者：
Chilkoti, Ashutosh
中科院分区：
文献类型：
--
作者：
Hucknall, Angus;Rangarajan, Srinath;Chilkoti, Ashutosh
抗蛋白质或“无污染”表面对于各种生物医学和生物技术应用具有极大的兴趣。本文简要介绍了抗蛋白质表面的研究进展，并介绍了利用表面引发聚合制备抗蛋白质污染表面的新方法。我们表明，聚合物刷合成的表面引发聚合，目前短的寡（乙二醇）侧链是异常耐蛋白质吸附和细胞粘附。由这些聚合物刷赋予的蛋白质和细胞抗性的重要性通过它们用作制造抗体微阵列的基底来说明，所述抗体微阵列在复杂流体如血清和血液中表现出飞摩尔检测极限，对中间洗涤步骤的要求放宽。该实施例强调了重要的一点，即由蛋白质抗性表面提供的背景噪声的减少可以极大地简化具有增加的灵敏度和实用性的超灵敏异质的基于表面的临床和蛋白质组学测定的开发。
Protein resistant or "non-fouling" surface are of great interest for a variety of biomedical and biotechnology applications. This article briefly reviews the development of protein resistant surface, followed by recent research on a new methodology to fabricate non-fouling surface by surface-initiated polymerization. We show that polymer brushes synthesized by surface-initiated polymerization that present short oligo(ethylene glycol) side chains are exceptionally resistant to protein adsorption and cell adhesion. The importance of the protein and cell resistance conferred by these polymer brushes is illustrated by their use as substrates for the fabrication of antibody microarrays that exhibit femtomolar limits of detection in complex fluids such as serum and blood with relaxed requirements for intermediate wash steps. This example highlights the important point that the reduction in background noise afforded by protein-resistant surface can greatly simply the development of ultrasensitive heterogeneous, surface-based clinical and proteomic assays with increased sensitivity and utility.