High-speed atomic force microscopy for observing dynamic biomolecular processes
High-speed atomic force microscopy for observing dynamic biomolecular processes
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DOI：
10.1002/jmr.843
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发表时间：
2007-11-01
影响因子：
2.7
通讯作者：
Yamashita, Hayato
中科院分区：
文献类型：
--
作者：
Ando, Toshio;Uchihashi, Takayuki;Yamashita, Hayato
原子力显微镜（AFM）在其捕获液体中生物样品的高分辨率图像的能力方面是独一无二的。这种能力将变得更有价值的生物科学，如果原子力显微镜另外获得高速成像的能力，因为“直接和实时可视化”是一个简单而强大的手段来了解生物分子的过程。传统的原子力显微镜需要一分钟以上的时间来捕捉图像，而生物分子过程通常发生在毫秒级或更短的时间尺度上。为了填补这一巨大的空白，在过去的十年中进行了各种努力。在这里，我们回顾这些过去的努力，描述了目前的状态的能力和限制的高速AFM，并讨论可能打破限制，并导致发展一个真正有用的高速AFM生物科学的可能性。版权所有（c）2007约翰威利父子有限公司。
The atomic force microscope (AFM) is unique in its capability to capture high-resolution images of biological samples in liquids. This capability will become more valuable to biological sciences if AFM additionally acquires an ability of high-speed imaging, because 'direct and real-time visualization' is a straightforward and powerful means to understand biomolecular processes. With conventional AFMs, it takes more than a minute to capture an image, while biomolecular processes generally occur on a millisecond timescale or less. In order to fill this large gap, various efforts have been carried out in the past decade. Here, we review these past efforts, describe the current state of the capability and limitations of high-speed AFM, and discuss possibilities that may break the limitations and lead to the development of a truly useful high-speed AFM for biological sciences. Copyright (c) 2007 John Wiley & Sons, Ltd.