The effect of Porphyromonas gingivalis on the gut microbiome of mice in relation to aging
The effect of Porphyromonas gingivalis on the gut microbiome of mice in relation to aging
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DOI：
10.1111/jre.13062
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发表时间：
2022-10-17
影响因子：
3.5
通讯作者：
Furuichi, Yasushi
中科院分区：
文献类型：
--
作者：
Giri, Sarita;Uehara, Osamu;Furuichi, Yasushi
背景和目的口腔细菌（包括牙龈卟啉单胞菌）移位到肠道已被证明会改变肠道微生物组。然而，牙龈卟啉单胞菌对与衰老有关的肠道微生物组的影响尚未得到证实。我们推测牙龈卟啉单胞菌随着年龄的增长对肠道环境有更大的不利影响。本研究旨在探讨牙龈卟啉单胞菌对老年小鼠肠道环境的影响。材料与方法将4周龄（幼龄）和76周龄（老龄）C57 BL/6 J小鼠分为4组：对照组-幼龄、对照组-老龄、牙龈卟啉单胞菌给药组-幼龄和牙龈卟啉单胞菌给药组。牙龈卟啉单胞菌每周口服给药三次，持续5周。在最后一次牙龈卟啉单胞菌给药后30天，进行16 S rRNA测序以研究肠道微生物组。评价肠连接屏障分子的mRNA和蛋白表达以及血清中炎性细胞因子IL-1 β和TNF-α的水平。结果在分类丰度、细菌多样性和预测的宏基因组功能方面，观察到组间肠道微生物组的显著差异。在施用牙龈卟啉单胞菌的老年小鼠中观察到有益细菌（例如阿克曼氏菌和梭菌科）的α多样性和丰度的显著降低。在给予牙龈卟啉单胞菌的老年小鼠中，肠道中Claudin-1和Claudin-2的mRNA和蛋白水平显著升高，而E-钙粘蛋白显著下调，IL-1 β和TNF-α的血清水平也是如此。结论牙龈卟啉单胞菌对老年小鼠肠道环境的影响较青年小鼠明显。
Background and Objective The translocation of oral bacteria, including Porphyromonas gingivalis, to the gut has been shown to alter gut microbiome. However, the effect of P. gingivalis on gut microbiome in relation to aging has not been demonstrated. We hypothesize that P. gingivalis has more detrimental effect on gut environment with increased age. The objective of this study is to investigate the effect of P. gingivalis on gut environment using aged mice. Materials and Methods C57BL/6J mice aged 4 weeks (young) or 76 weeks (old) were divided into four groups: control-young, control-old, P. gingivalis-administered young, and P. gingivalis-administered old. P. gingivalis was orally administered thrice weekly for 5 weeks. At 30 days after the last P. gingivalis administration, 16S rRNA sequencing was performed to study the gut microbiome. The mRNA and protein expression of intestinal junctional barrier molecules and the levels of the inflammatory cytokines IL-1 beta and TNF-alpha in the serum were evaluated. Results Significant differences in the gut microbiomes between the groups, in terms of taxonomic abundance, bacterial diversity, and predicted metagenome function, were observed. A significant reduction in the alpha diversity and in the abundance of beneficial bacteria, such as Akkermansia and Clostridiaceae, in the P. gingivalis-administered old mice was observed. The mRNA and protein levels of Claudin-1 and Claudin-2 in the intestine were significantly elevated, while E-cadherin was significantly downregulated in the P. gingivalis-administered old mice, as were the serum levels of IL-1 beta and TNF-alpha. Conclusion The effect of P. gingivalis on the gut environment is more pronounced in old mice than in young mice.