Urinary 8-hydroxydeoxyguanosine is elevated in patients with nephrolithiasis
Urinary 8-hydroxydeoxyguanosine is elevated in patients with nephrolithiasis
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DOI：
10.1007/s00240-007-0098-0
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发表时间：
2007-08-01
影响因子：
--
通讯作者：
Tosukhowong, Piyaratana
中科院分区：
文献类型：
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作者：
Boonla, Chanchai;Wunsuwan, Rattiporn;Tosukhowong, Piyaratana
8-羟基脱氧鸟苷(8-OHdG)是一种氧化修饰的鸟苷，广泛应用于多种疾病的DNA氧化损伤标记物。本研究旨在测定肾结石患者尿8-OHdG，并评价其临床意义。研究对象为36例肾结石患者和30例健康人。测定24 h尿量、肌酐、丙二醛、乙酰-N-氨基葡萄糖苷酶(NAG)活性和蛋白含量。用竞争性酶联免疫吸附试验测定尿8OHdG。用傅里叶变换红外光谱分析了石材的矿物组成。肾结石患者尿8-OHdG排泄量明显高于健康对照组。草酸钙、鸟粪石和尿酸结石患者的尿8-OHdG水平比较无显著差异。尿NAG活性与尿8OHdG呈正相关。多元线性回归分析显示，尿NAG活性是尿8OHdG水平的独立预测因子。受试者工作特性分析表明，尿8-OHdG试验对肾结石的诊断是合适的。以10 mU肌酐为界值，8-OHdG试验具有较高的特异性(96.67%)和阳性预测值(91.67%)。总之，这是首次报道肾结石患者尿8-OHdG排泄量升高，表明DNA氧化损伤增加。肾小管损伤增加与尿8-OHdG升高独立相关。尿8-OHdG水平升高与代谢谱相结合可能有助于识别结石形成的风险人群。
8-hydroxydeoxyguanosine (8-OHdG) is an oxidatively modified guanosine, which has been widely used as an oxidative DNA damage marker in various diseases. The present study aimed to determine urinary 8-OHdG in nephrolithiasis patients and evaluate its clinical significance. Thirty-six nephrolithiasis patients and 30 healthy subjects were recruited. Urine volume, creatinine, malondialdehyde, fl-N-acetylglucosaminidase (NAG) activity and proteins were measured in 24 h urine samples. Urinary 8OHdG was determined by competitive enzyme-linked immunosorbent assay. Mineral composition of stones was analyzed using Fourier-transformed infrared spectroscopy. Nephrolithiasis patients excreted urinary 8-OHdG significantly higher than healthy controls. Urinary 8-OHdG levels compared among patients with calcium oxalate, struvite and uric acid stones were insignificantly different. The urinary NAG activity correlated positively with urinary 8OHdG. Multiple linear regression showed that urinary NAG activity was an independent predictor of urinary 8OHdG level. Receiver operating characteristic analysis revealed that the urinary 8-OHdG test was adequate for diagnosing nephrolithiasis. At 10 mu glg creatinine cutoff, the 8-OHdG test imparted high specificity (96.67%) and a positive predictive value (91.67%). In conclusion, this is the first report of elevated urinary 8-OHdG excretion in nephrolithiasis patients indicating increased oxidative DNA damage. Increased renal tubular damage was independently associated with elevated urinary 8-OHdG. Elevated urinary 8-OHdG levels adjunct with metabolic profile may be useful for identifying people at risk of stone development.