Effects of estradiol on glucoprivic transactivation of catecholaminergic neurons in the female rat caudal brainstem
Effects of estradiol on glucoprivic transactivation of catecholaminergic neurons in the female rat caudal brainstem
复制标题
DOI：
10.1159/000054655
复制
发表时间：
2001-06-01
影响因子：
4.1
通讯作者：
Sylvester, PW
中科院分区：
文献类型：
--
作者：
Briski, KP;Marshall, ES;Sylvester, PW
卵巢类固醇雌二醇（E）可调节急性缺糖后的高吞噬和下丘脑神经内分泌反应。通过血糖减少症观察到的后脑外侧网状核、孤束核和最后区（AP）中的儿茶酚胺能（CA）神经元的基因组激活支持其在介导脑内代谢挑战的调节效应的途径中的潜在功能。这些细胞的E受体表达表明，它们的活性可能是敏感的糖皮质激素调节过程中。本研究探讨了葡萄糖抗代谢物2-脱氧-D-葡萄糖（2DG）对尾侧脑干CA神经元转录激活的作用。卵巢切除大鼠皮下植入。含E（30或250 μ g/ml）或芝麻油的硅橡胶胶囊，7天后腹腔注射400 mg 2DG/kg或生理盐水。抗代谢药给药后，C-1/A（1）、C-2、C-3、A（2）、A（5）和A（6）细胞组和AP中的酪氨酸羟化酶（TH）免疫反应（-ir）神经元共标记Fos，而溶剂注射导致这些细胞的核染色可忽略不计。除A（2）、A（6）和AP细胞外，E-和芝麻油植入组之间这些脑部位的Fos和TH-/Fos-ir阳性神经元的平均数量没有差异。与低类固醇剂量或芝麻油相比，植入高E剂量的大鼠中对2DG做出反应而表达Fos的TH阳性A（2）和A（6）神经元数量显着更多。这些结果表明，离散后脑CA神经元群体内的细胞Fos标记的幅度根据循环E水平而变化。这些研究结果表明，E可能发挥潜在的调节作用glucoprivic激活的Fos刺激/转录级联反应和随之而来的补偿性基因组反应内的特定领域的雌性大鼠尾侧脑干。版权所有（C）2001 S. Karger AG，巴塞尔。
Hyperphagic and hypothalamic neuroendocrine responses to acute glucose deprivation are modified by the ovarian steroid estradiol (E). Observations of genomic activation of catecholaminergic (CA) neurons in the hindbrain lateral reticular nucleus, nucleus of the solitary tract, and area postrema (AP) by glucopenia support their potential function in pathways mediating regulatory effects of this metabolic challenge within the brain. Expression of E receptors by these cells suggests that their activity may be sensitive to steroid modulation during glucopenia. The present studies investigated the role of E on transcriptional activation of caudal brainstem CA neurons by the glucose antimetabolite, 2-deoxy-D-glucose (2DG). Ovariectomized rats were implanted with s.c. Silastic capsules containing E (30 or 250 mug/ml) or sesame oil, and injected i.p. 7 days later with 400 mg 2DG/kg or saline. Tyrosine hydroxy[ase (TH)-immunore-active (-ir) neurons in the C-1/A(1), C-2, C-3, A(2), A(5), and A(6) cell groups and AP were colabeled for Fos following antimetabolite administration, whereas vehicle injection resulted in negligible nuclear staining of these cells. With the exception of A(2), A(6), and AP cells, mean numbers of Fos- and TH-/Fos-ir-positive neurons in these brain sites did not differ between E- and sesame oil-implanted groups. Numbers of TH-positive A(2) and A(6) neurons that expressed Fos in response to 2DG were significantly greater in rats implanted with the high E dose vs. either the low steroid dose or sesame oil. These results show that the magnitude of cellular Fos labeling within discrete hindbrain CA neuron populations varies in accordance with circulating E levels. These findings suggest that E may exert potential modulatory effects on glucoprivic activation of the Fos stimulus/transcription cascade and consequent compensatory genomic responses within specific areas of the female rat caudal brainstem. Copyright (C) 2001 S. Karger AG, Basel.