Systemic necrosis in tomato induced by a Japanese isolate of rehmannia mosaic virus in a temperature-sensitive manner
Systemic necrosis in tomato induced by a Japanese isolate of rehmannia mosaic virus in a temperature-sensitive manner
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DOI：
10.1111/ppa.13006
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发表时间：
2019-06-01
期刊：
影响因子：
2.7
通讯作者：
Hikichi, Y.
中科院分区：
文献类型：
--
作者：
Hamada, T.;Mise, K.;Hikichi, Y.
日本辣椒花叶病毒分离物（ReMV-J）最初是从日本的辣椒中分离得到的。不携带抗病毒基因的病株辣椒叶片出现花叶症状。随着症状的进展，受感染的植物发展为明显的叶片坏死和严重的落叶，随后是茎坏死和果实变形。此外，ReMV-J系统性地感染番茄，据报道，当在20摄氏度下孵育时，这导致番茄中的斑驳。这项研究发现，ReMV-J诱导番茄系统性坏死-除了品种Micro-Tom -当在25摄氏度下孵育时。为了确定参与诱导番茄温度敏感性系统坏死的病毒因子，构建了ReMV-J与番茄花叶病毒的基因间重组体。所有重组病毒对番茄的系统侵染与ReMV-J相似，携带ReMV-J运动蛋白（MP）基因的重组病毒引起番茄系统坏死。在20 ℃下孵育显著降低了具有ReMV-J MP基因的重组病毒诱导全身性坏死的能力。因此，ReMV-J MP基因参与了大多数番茄品种的系统性坏死的温度敏感性诱导。这项研究是第一次报告的MP基因的参与，在温度敏感的诱导系统性坏死，由番茄花叶病毒对番茄花叶病毒敏感。
The Japanese isolate of rehmannia mosaic virus (ReMV-J) was initially isolated from chilli pepper in Japan. The leaves of diseased chilli pepper plants, which hold no tobamovirus-resistance genes, have mosaic symptoms. As the symptoms progress, the infected plants develop prominent leaf necrosis and severe leaf fall, followed by stem necrosis and fruit distortion. Additionally, ReMV-J systematically infects tomato and this reportedly leads to mottle in tomato when incubated at 20 degrees C. This study found that ReMV-J induces systemic necrosis of tomato - except for cultivar Micro-Tom - when incubated at 25 degrees C. To determine the virus factor involved in the induction of temperature-sensitive systemic necrosis on tomato, intergenic recombinants between ReMV-J and tomato mosaic virus were constructed. All recombinant viruses systemically infected tomato similar to ReMV-J. Recombinant viruses with the movement protein (MP) gene of ReMV-J induced systemic necrosis on tomato. Incubation at 20 degrees C significantly reduced the ability of recombinant viruses with the MP gene of ReMV-J to induce systemic necrosis. Thus, the ReMV-J MP gene is involved in the temperature-sensitive induction of systemic necrosis on most tomato cultivars tested. This study is the first to report the involvement of the MP gene in temperature-sensitive induction of systemic necrosis by tobamovirus on tobamovirus-susceptible tomato.