Biofilm removal mediated by Salmonella phages from chicken-related sources
Biofilm removal mediated by Salmonella phages from chicken-related sources
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沙门氏菌噬菌体介导的鸡相关来源的生物膜去除
DOI：
10.1016/j.fshw.2023.02.044
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发表时间：
2023-09-01
影响因子：
7
通讯作者：
Wang，Huhu
中科院分区：
文献类型：
--
作者：
Ning，Zhenzhen;Zhang，Lingling;Wang，Huhu
沙门氏菌及其生物膜形成是食源性疾病爆发和交叉污染的主要原因。该研究的目的是调查沙门氏菌噬菌体作为生物膜去除替代技术的潜力。在这项工作中，从养鸡场和屠宰场分离出 21 种沙门氏菌噬菌体，并对具有最广谱的噬菌体 (CW1) 进行了表征。完整的基因组序列分析表明，噬菌体CW1的基因组由41763 bp、58个开放阅读框（ORF）和穴蛋白-内溶素系统组成，不编码任何毒力或溶源性。建立了由 CW1（最广谱为 70.49%）和 CW11、M4 和 M10（具有超过 67.11% 的高裂解活性）组成的噬菌体混合物。用混合物处理使正在发育的生物膜和成熟生物膜中的细胞分别减少了 0.79 lg(CFU/cm2) 和 0.4 lg(CFU/cm2)。通过共焦激光扫描显微镜和扫描电子显微镜观察到更多的死细胞和分散的细胞外聚合物（EPS）。拉曼分析发现碳水化合物和蛋白质是分散EPS的识别受体。这一发现表明，这种噬菌体混合物在肉类加工过程中沙门氏菌生物膜的灭菌方面具有潜在的应用。
Salmonellaand their biofilm formation are the primary bacterial causes of foodborne outbreaks and cross-contamination. The objective of the study was to investigate the potential ofSalmonellaphages as an alternative technology for biofilm removal. In this work, 21Salmonellaphages were isolated from a chicken farm and slaughter plant and the phage (CW1) with the broadest spectrum was characterized. Complete genome sequence analysis revealed that the genomes of phage CW1 is composed of 41763 bp with 58 open reading frames (ORFs) and a holin-endolysin system and it does not encode any virulence or lysogeny. A phage cocktail consisted of CW1 (with the broadest spectrum of 70.49 %) and CW11, M4 and M10 (with a high lytic activity of more than 67.11 %) was established. Treatment with the cocktail reduced the cells in the developing biofilm and mature biofilm by 0.79 lg(CFU/cm2) and 0.4 lg(CFU/cm2), respectively. More dead cells and scattered extracellular polymeric substances (EPS) were observed by confocal laser scanning microscopy and scanning electron microscopy. Raman analysis found that carbohydrates and proteins were the identification receptors for scattered EPS. This finding suggests that this phage cocktail has potential applications for the sterilization ofSalmonellabiofilm during meat processing.