HEPATITIS-C VIRUS-ANTIBODIES IN CHRONIC ACTIVE HEPATITIS - PATHOGENETIC FACTOR OR FALSE-POSITIVE RESULT
HEPATITIS-C VIRUS-ANTIBODIES IN CHRONIC ACTIVE HEPATITIS - PATHOGENETIC FACTOR OR FALSE-POSITIVE RESULT
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DOI：
10.1016/0140-6736(90)90870-b
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发表时间：
1990-03-31
期刊：
影响因子：
168.9
通讯作者：
WILLIAMS, R
中科院分区：
文献类型：
--
作者：
MCFARLANE, IG;SMITH, HM;WILLIAMS, R
应用酶联免疫吸附试验（Ortho-HCVELISA）检测53例自身免疫性慢性活动性肝炎（AI-CAH）患者143份血清中的丙型肝炎病毒（HCV）抗体。测定中的光密度（OD）值与血清球蛋白（r = 0.8846，p < 0.0005）和IgG（r = 0.6281，p < 0.0005）浓度密切相关，但与免疫抑制剂治疗无关。31例活动期患者中20例（65%）OD值阳性，22例缓解期患者中仅1例（5%）OD值阳性（p < 0.0005）。阳性结果与活动性疾病和高血清球蛋白水平之间的关联通过对6例患者的系列研究得到证实。与此相反，31例原发性胆汁性肝硬化患者和24例非肝病患者中仅有2例IgG浓度高，这些对照组显示OD值与血清球蛋白或IgG之间无相关性。研究结果表明，来自AI-CAH患者的血清可能含有在测定中产生假阳性结果的成分。
An enzyme immunoassay (Ortho-HCV ELISA) for antibodies against the hepatitis C virus (HCV) was used to test 143 serum samples from 53 patients with autoimmune chronic active hepatitis (AI-CAH). Optical density (OD) values in the assay correlated closely with serum globulin (r = 0.8846, p < < 0.0005) and IgG (r = 0.6281, p < 0.0005) concentrations but not with immunosuppressant therapy. OD values were positive in 20 (65%) of 31 with active disease and in only 1 (5%) of 22 in remission (p < 0.0005). The association between positive results and active disease and high serum globulin levels was confirmed by serial studies in 6 of the patients. In contrast, none of 31 patients with primary biliary cirrhosis and only 2 of 24 with non-hepatic disorders asosciated with high IgG concentrations were positive, and these controls showed no correlation between OD values and serum globulins or IgG. The findings suggest that serum from AI-CAH patients may contain a component that gives false-positive results in the assay.