IONIZING-RADIATION ACTIVATES TRANSCRIPTION OF THE EGR1 GENE VIA CARG ELEMENTS
IONIZING-RADIATION ACTIVATES TRANSCRIPTION OF THE EGR1 GENE VIA CARG ELEMENTS
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DOI：
10.1073/pnas.89.21.10149
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发表时间：
1992-11-01
影响因子：
11.1
通讯作者：
KUFE, DW
中科院分区：
文献类型：
--
作者：
DATTA, R;RUBIN, E;KUFE, DW
本文研究了电离辐射对早期生长反应1(Egr1)基因的调控作用。人HL-525细胞受X射线照射后，Egr1基因表达水平升高。核连续分析表明，这种作用至少部分与Egr1基因转录的激活有关。通过对Egr1启动子的缺失分析来确定对电离辐射诱导信号的响应序列。结果表明，Egr1基因的X射线诱导性是由包含6个血清反应或CC(A+T富含)6GG(Carg)基序的区域决定的。进一步的分析证实，包含三个远端或上游CAG元件的区域在X射线反应中是起作用的。此外，该区域对最小胸苷激酶基因启动子具有X射线诱导性。综上所述，这些结果表明电离辐射通过Carg元件诱导Egr1转录。
The present studies have examined the effects of ionizing radiation on control of the early growth response 1 (EGR1) gene. Exposure of human HL-525 cells to x-rays was associated with increases in EGR1 mRNA levels. Nuclear run-on assays showed that this effect is related at least in part to activation of EGR1 gene transcription. Sequences responsive to ionizing radiation-induced signals were determined by deletion analysis of the EGR1 promoter. The results demonstrate that x-ray inducibility of the EGR1 gene is conferred by a region containing six serum response or CC(A+T-rich)6GG (CArG) motifs. Further analysis confirmed that the region encompassing the three distal or upstream CArG elements is functional in the x-ray response. Moreover, this region conferred x-ray inducibility to a minimal thymidine kinase gene promoter. Taken together, these results indicate that ionizing radiation induces EGR1 transcription through CArG elements.