Experimental development of a new protocol for extraction and characterization of microplastics in fish tissues: First observations in commercial species from Adriatic Sea
Experimental development of a new protocol for extraction and characterization of microplastics in fish tissues: First observations in commercial species from Adriatic Sea
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DOI：
10.1016/j.marenvres.2015.06.014
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发表时间：
2015-10-01
影响因子：
3.3
通讯作者：
Regoli, Francesco
中科院分区：
文献类型：
--
作者：
Avio, Carlo Giacomo;Gorbi, Stefania;Regoli, Francesco
在过去十年中，海洋环境中微塑料的存在引起了科学界的兴趣。几种生物可以摄入微塑料，对消化道、呼吸系统和运动附属物有潜在的不利影响。然而，仍然缺乏这种微粒在野生生物体中的组织积累和转移的明确证据，部分地受到从生物样品分离和表征方案的技术困难的阻碍。在这项工作中，我们比较了一些现有方法的有效性，并优化了一种新的方案，允许从鱼类组织中提取微塑料的提取率在78%到98%之间，具体取决于聚合物的大小。FT-IR分析证实，浸提程序不会影响颗粒特性。在实验室条件下暴露于聚苯乙烯和聚乙烯的鲻鱼（Mugil cephalus）上进一步验证了该方法;在胃和肝脏中分离并定量了颗粒，并通过组织学分析证实了其在肝组织中的存在。初步表征揭示了微塑料在沿着亚得里亚海采集的各种鱼类中的存在和分布。FT-IR分析表明，聚乙烯是鱼胃中的主要聚合物（65%）。总体结果证实了新开发的方法是检测和量化海洋生物群中微塑料的可靠方法。(C)2015爱思唯尔有限公司版权所有。
The presence of microplastics in the marine environment has raised scientific interest during the last decade. Several organisms can ingest microplastics with potentially adverse effects on the digestive tract, respiratory system and locomotory appendages. However, a clear evidence of tissue accumulation and transfer of such microparticles in wild organisms is still lacking, partially hampered by technical difficulties in isolation and characterization protocols from biological samples. In this work, we compared the efficacy of some existing approaches and we optimized a new protocol allowing an extraction yield of microplastics from fish tissues ranging between 78% and 98%, depending on the polymer size. FT-IR analyses confirmed that the extraction procedure did not affect the particles characteristics. The method was further validated on the fish mullet, Mugil cephalus, exposed under laboratory conditions to polystyrene and polyethylene; the particles were isolated and quantified in stomach and liver, and their presence in the hepatic tissue was confirmed also by histological analyses. A preliminary characterization revealed the presence and distribution of microplastics in various fish species collected along the Adriatic Sea. FT-IR analyses indicated polyethylene as the predominant polymer (65%) in the stomach of fish. The overall results confirmed the newly developed method as a reliable approach to detect and quantify microplastics in the marine biota. (C) 2015 Elsevier Ltd. All rights reserved.