Long-term effects of chronic ethanol on muscarinic receptor binding in rat brain.
Long-term effects of chronic ethanol on muscarinic receptor binding in rat brain.
复制标题
慢性乙醇对大鼠脑中毒蕈碱受体结合的长期影响。
DOI：
10.1111/j.1530-0277.1996.tb01706.x
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发表时间：
1996
期刊：
影响因子：
--
通讯作者：
Anderson,KJ
中科院分区：
文献类型：
--
作者：
Rothberg,BS;Hunter,BE;Walker,DW;Anderson,JF;Anderson,KJ
通过定量放射自显影法研究了长期乙醇处理（CET）对大鼠毒蕈碱乙酰胆碱受体（mAChR）结合特性的影响，大鼠持续摄入含乙醇的液体饲料28周，并在收获组织前停止摄入乙醇8周。对照组接受相同的饮食，其中蔗糖等热量取代乙醇。在海马CA1区、齿状回、新皮质、纹状体或丘脑中，放射性标记的mAChR拮抗剂奎宁环二苯甲酸酯（[3H]QNB）的最大结合没有减少，表明CET在这些区域中没有导致显著的mAChR损失。结合亲和力的胆碱能激动剂卡巴胆碱mAChRs不受CET在这些地区的每一个，通过竞争性置换[3H]QNB标记。这些结果表明，CET诱导的脑胆碱能反应功能缺陷不是由于CET对mAChR结合特性的直接影响。
Effects of chronic ethanol treatment (CET) on muscarinic acetylcholine receptor (mAChR) binding properties were investigated via quantitative autoradiography in rats maintained on an ethanol‐containing liquid diet for 28 weeks and withdrawn from ethanol for 8 weeks before harvesting of tissues. Controls received an identical diet in which sucrose was substituted isocalorically for ethanol. Maximal binding of the radiolabeled mAChR antagonist quinuclidinyl benzilate ([3H]QNB) was not reduced in hippocampal area CA1, dentate gyrus, neocortex, striatum, or thalamus, suggesting that CET results in no significant mAChR loss in these regions. Binding affinities of the cholinergic agonist carbachol to mAChRs were unaffected by CET in each of these regions, as determined by competitive displacement of [3H]QNB labeling. These results suggest that CET‐induced functional deficits in brain cholinergic responses are not due to direct effects of CET on mAChR binding properties.