ANALYSIS OF MOLECULAR-SPECIES OF PLANT POLAR LIPIDS BY HIGH-PERFORMANCE AND GAS-LIQUID CHROMATOGRAPHY
ANALYSIS OF MOLECULAR-SPECIES OF PLANT POLAR LIPIDS BY HIGH-PERFORMANCE AND GAS-LIQUID CHROMATOGRAPHY
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DOI：
10.1016/s0031-9422(00)80752-9
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发表时间：
1985-01-01
期刊：
影响因子：
3.8
通讯作者：
MAZLIAK, P
中科院分区：
文献类型：
--
作者：
DEMANDRE, C;TREMOLIERES, A;MAZLIAK, P
通过2步HPLC [高效液相色谱法]，使用反相色谱柱将马铃薯块茎和烟叶的总脂质提取物分离为脂质类。首先使用从100%溶液A（异丙醇-己烷，4：3）至100%溶液B（异丙醇-己烷-水，8：6：1.5）的2种混合溶剂的程序线性梯度洗脱20 min;然后使用纯溶液B以等度模式洗脱色谱柱20 min以上。可以收集来自两种植物组织的主要极性脂质（MGDG、DGDG、PC、PE、PG），并在具有C18柱的简化HPLC系统上进一步分离成组分分子种类，所述C18柱以等度模式用极性溶剂洗脱。在35分钟内实现分子种类分离;通过GLC分析附着的脂肪酸进行定量。因此，在每种脂质类别中清楚地鉴定出三至五种主要分子种类。在马铃薯块茎中，磷脂（PC，PE）18：2/18：2种类占优势。在烟草叶中，6种双键种类（18：3/18：3和16：3/18：3）在半乳糖脂中占主导地位; PC含有更多的分子种类，这些分子种类根据其不饱和度而变化（从18：3/18：3到16：0/18：2）。只有某些PG分子种类含有Δ 3-反式-十六碳烯酸。
Total lipid extracts from potato tubers and tobacco leaves are separated into lipid classes by 2 step HPLC [high performance liquid chromatography] using a silicic column. Elution is first performed for 20 min with a programmed linear gradient of 2 mixed solvents running from 100% of solution A (isopropanol-hexane, 4:3) to 100% of solution B (isopropanol-hexane-water, 8:6:1.5); the column is then eluted with pure solution B in an isocratic mode for 20 min more. The main polar lipids (MGDG, DGDG, PC, PE, PG) from both plant tissues can be collected and further separated into component molecular species on a simplified HPLC system with a C18 column eluted in an isocratic mode with a polar solvent. Molecular species separations are achieved within 35 min; quantifications are made through GLC analysis of attached fatty acids. Three to five main molecular species are thus clearly identified in each lipid class. In potato tuber, phospholipids (PC, PE) 18:2/18:2 species are predominant. In tobacco leaf, 6 double bond species (18:3/18:3 and 16:3/18:3) are predominant in galactolipids; PC contains a greater number of molecular species varying by their degree of unsaturation (from 18:3/18:3 to 16:0/18:2). Only certain molecular species of PG contain .DELTA.3-trans-hexadecenoic acid.