Expression of the thrombin receptor PAR-1 correlates with tumour cell differentiation of pancreatic adenocarcinoma in vitro
Expression of the thrombin receptor PAR-1 correlates with tumour cell differentiation of pancreatic adenocarcinoma in vitro
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凝血酶受体 PAR-1 的表达与体外胰腺腺癌的肿瘤细胞分化相关
DOI：
--
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发表时间：
2004
影响因子：
4
通讯作者：
E. Neugebauer
中科院分区：
文献类型：
--
作者：
C. Rudroff;S. Seibold;R. Kaufmann;Cecilia Cu Zetina;Kathrin Reise;U. Schäfer;A. Schneider;M. Brockmann;J. Scheele;E. Neugebauer
胰腺癌患者常因凝血酶生成过多而形成血栓。然而，凝血酶对胰腺癌的作用仍然知之甚少。凝血酶受体PAR-1负责凝血酶的细胞效应。PAR-1在体外不同实体肿瘤的进展中起重要作用。在乳腺癌中，PAR-1的表达水平与浸润性相关。我们的目的是将PAR-1 mRNA和蛋白表达水平与胰腺组织和癌细胞的分化程度联系起来。正常胰腺上皮未检出PAR-1蛋白。免疫荧光染色法分析胰腺癌细胞系中PAR-1蛋白的表达与分化程度相关。Western Blot定量分析PAR-1蛋白表达证实了这些观察结果。PAR-1 mRNA表达分析显示，与胰腺癌组织和胰腺癌细胞系MIA PaCa-2相比，健康胰腺中的PAR-1 mRNA表达水平较低。PAR-1 mRNA的水平在不同的胰腺癌细胞系之间差异高达25倍。在体内和体外健康胰腺组织和胰腺癌中，PAR-1蛋白和mRNA表达的显著差异强调了PAR-1在胰腺癌进展中的假定作用。
Patients with pancreatic cancer frequently suffer from thrombosis due to excess thrombin generation. Yet, the effects of thrombin on pancreatic cancer are still poorly understood. The thrombin receptor PAR-1 is responsible for cellular effects of thrombin. PAR-1 plays an important role in the progression of different solid tumours in vitro. In breast cancer the level of PAR-1 expression correlates with invasiveness. Our aim was to correlate PAR-1 mRNA and protein expression level with the grade of differentiation of pancreatic tissue and cancer cell lines. PAR-1 protein was not detectable in the epithelium of healthy pancreas. Analysis of PAR-1 protein expression by immunofluorescence staining of pancreatic cancer cell lines revealed a correlation to the grade of differentiation. Quantitative analysis of PAR-1 protein expression by Western Blot analysis confirmed these observations. Analysis of PAR-1 mRNA expression showed low levels in healthy pancreas compared to pancreatic cancer tissue and the pancreatic cancer cell line MIA PaCa-2. The level of PAR-1 mRNA differed up to 25 fold between the respective pancreatic cancer cell lines. The eminent differences in PAR-1 expression, both protein and mRNA, between healthy pancreatic tissue and pancreatic cancer in vivo and in vitro emphasise the putative role of PAR-1 in pancreatic cancer progression.
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影响因子：
11.2
作者：
M. Nierodzik;F. Kajumo;S. Karpatkin
通讯作者：
M. Nierodzik;F. Kajumo;S. Karpatkin
培养的人内皮细胞中凝血酶诱导的前列环素产生与肌醇三磷酸和胞质游离钙水平之间的相关性。
DOI：
--
发表时间：
1987
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Jaffe,EA;Grulich,J;Weksler,BB;Hampel,G;Watanabe,K
通讯作者：
Watanabe,K
影响因子：
11.2
作者：
Lakshmi Kamath;Ahou Meydani;F. Foss;A. Kuliopulos
通讯作者：
Lakshmi Kamath;Ahou Meydani;F. Foss;A. Kuliopulos
凝血酶信号传导的分子机制。
影响因子：
3.6
作者：
Coughlin,SR
通讯作者：
Coughlin,SR
高度纯化的人 α-凝血酶促进 BALB/c 3T3 细胞的形态转化。
影响因子：
4.7
作者：
Morris,DL;WardJr,JB;Nechay,P;WhortonJr,EB;Fenton2nd,JW;Carney,DH
通讯作者：
Carney,DH