On-line coupling of immunoaffinity-based solid-phase extraction and gas chromatography for the determination of s-triazines in aqueous samples
On-line coupling of immunoaffinity-based solid-phase extraction and gas chromatography for the determination of s-triazines in aqueous samples
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DOI：
10.1016/s0021-9673(98)00932-7
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发表时间：
1999-01-15
影响因子：
4.1
通讯作者：
Brinkman, UAT
中科院分区：
文献类型：
--
作者：
Dall端ge, J;Hankemeier, T;Brinkman, UAT
研究了基于免疫亲和的固相萃取 (IASPE) 与气相色谱 (GC) 在线耦合测定微污染物的潜力，重点是系统中基于免疫亲和的 SPE 和 GC 部分的接口。含有固定化抗体的柱通过反相柱（共聚物吸附剂）连接到气相色谱仪。在免疫亲和柱上痕量富集分析物后，通过酸性缓冲液将它们解吸并重新收集在反相柱上。用氮气净化和干燥后，使用乙酸乙酯通过柱上接口以部分并发溶剂蒸发模式进行解吸并转移至 GC。免疫亲和盒中使用的抗体是针对莠去津产生的；使用几种均三嗪作为测试化合物。结构与莠去津相似的三嗪显示出定量回收。作为一种应用，免疫亲和 SPE-GC 用于分析河流、废水和橙汁。该系统的选择性使得非选择性火焰离子化检测 (FID) 可用于检测这些复杂基质中的目标分析物。对于 10 ml 水样，FID 的检测限为 15-25 ng/l，氮磷检测的检测限约为 1.5 ng/l。 (C) 1999 Elsevier Science B.V. 保留所有权利。
The potential of immunoaffinity-based solid-phase extraction (IASPE) coupled on-line to gas chromatography (GC) for the determination of micropollutants was studied with emphasis on the interfacing of the immunoaffinity-based SPE and GC parts of the system. The cartridge containing the immobilized antibodies was coupled to the gas chromatograph via a reversed-phase cartridge (copolymer sorbent). After trace enrichment of the analytes on the immunoaffinity cartridge, they were desorbed and recollected on the reversed-phase cartridge by means of an acidic buffer. After clean-up and drying with nitrogen, desorption and transfer to the GC was done with ethyl acetate via an on-column interface in the partially concurrent solvent evaporation mode. The antibodies used in the immunoaffinity cartridge were raised against atrazine; several s-triazines were used as test compounds. Triazines that were structurally similar to atrazine, showed quantitative recovery. As an application, immunoaffinity SPE-GC was used for the analysis of river and waste water and orange juice. The selectivity of the system was such that non-selective flame ionization detection (FID) could be used to detect the analytes of interest in these complex matrices. The detection limits for 10-ml water samples were 15-25 ng/l for FID and about 1.5 ng/l for the nitrogen-phosphorus detection. (C) 1999 Elsevier Science B.V. All rights reserved.