Differential expression and subcellular localization for subunits of cAMP-dependent protein kinase during ram spermatogenesis.
Differential expression and subcellular localization for subunits of cAMP-dependent protein kinase during ram spermatogenesis.
复制标题
DOI：
10.1083/jcb.109.3.1195
复制
发表时间：
1989-09
影响因子：
7.8
通讯作者：
Weinman, S
中科院分区：
文献类型：
--
作者：
Pariset, C;Feinberg, J;Dacheux, J L;Oyen, O;Jahnsen, T;Weinman, S
在不同的公羊生殖细胞中，已经研究了cAMP依赖蛋白激酶的RIα、RIIα和Cα亚基的mRNAs的表达。在生殖细胞中观察到的特异性RIα、RIIα和CαmRNAs的大小分别为1.6kb、2.0 kb和2.6kb。RIα和Cα基因主要在初级精母细胞中表达。在早期精子细胞中占主导地位的减数分裂后表达是RIIαmRNA所独有的。用免疫金电子显微镜观察RI、RIIα和C亚基在绵羊精子细胞和附睾精子细胞器中的定位。在精子细胞中，RI、RIIα和C主要存在于形成的顶体中，少量存在于细胞核中。在精子附睾体成熟过程中，蛋白激酶从顶体消失，并在顶体顶端、运动器和膜网络等多种精子功能区检测到。目前对cAMP依赖的蛋白激酶亚基的研究支持特定功能依附于不同亚基的概念，因为它在生殖细胞中显示了不同的表达和不同的亚细胞定位。
The expression of mRNAs for the RI alpha, RII alpha, and C alpha subunits of cAMP-dependent protein kinase has been studied in different ram germ cells. The sizes of the specific RI alpha, RII alpha, and C alpha mRNAs, observed in germ cells were 1.6, 2.0, and 2.6 kb, respectively. RI alpha and C alpha mRNAs were mainly expressed in primary spermatocytes. A postmeiotic expression predominating in early spermatids was unique to RII alpha mRNA. The location of RI, RII alpha, and C subunits in well-defined organelles of ram spermatids and epididymal sperm was assessed by immunogold electron microscopy. In spermatids, RI, RII alpha, and C were essentially present in the forming acrosome and, to a lesser extent, in the nucleus. During sperm epididymal maturation, the protein kinases disappeared from the acrosome and were detected in a variety of sperm functional areas, such as the tip of the acrosome, the motility apparatus, and the membrane network. The present study on subunits of cAMP-dependent protein kinase supports the concept that specific functions are attached to the different subunits in that it shows differential expression and differential subcellular localization in germ cells.