Electrospinning biomedical nanocomposite fibers of hydroxyapaite/poly(lactic acid) for bone regeneration
Electrospinning biomedical nanocomposite fibers of hydroxyapaite/poly(lactic acid) for bone regeneration
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DOI：
10.1002/jbm.a.30866
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发表时间：
2006-12-01
影响因子：
4.9
通讯作者：
Knowles, J. C.
中科院分区：
文献类型：
--
作者：
Kim, Hae-Won;Lee, Hae-Hyoung;Knowles, J. C.
生物陶瓷-生物聚合物纳米复合材料纤维基质的开发在骨再生和组织工程领域具有广阔的应用前景。然而，为了生产结构均匀的电纺丝纤维，陶瓷粉的细度和悬浮稳定性是至关重要的。在此，我们开发了一种新的方法，将生物陶瓷羟基磷灰石（HA）悬浮在生物聚合物聚乳酸（PLA）中。该策略是在亲水性HA粉末和疏水性氯仿溶解PLA之间引入表面活性剂羟基甾酸（HSA）。羟基磷灰石纳米粉末在羟基磷灰石中有效分散，并与聚乳酸混合均匀。成功制备了直径约为1-2 μ m的连续均匀纤维，具有发育良好的HA纳米粉末-分散PLA纳米复合结构。最初的细胞实验显示，在培养7天后，细胞附着和增殖良好，碱性磷酸酶的表达增强。HA-PLA纳米复合纤维可能在组织工程应用中有潜在的用途，特别是作为骨生长的三维基质。(c) 2006 Wiley期刊公司
Development of fibrous matrices of bioceramic-biopolymer nanocomposite offers great potential in the field of bone regeneration and tissue engineering. However, in order to produce electrospun fibers with homogeneous structure, it is essential for the ceramic powder to be fine and to remain stable in suspension. Herein, we developed a novel method whereby the bioceramic hydroxyapatite (HA) was kept in suspension in biopolymer poly(lactic acid) (PLA). The strategy was to introduce a surfactant hydroxysteric acid (HSA) between the hydrophilic HA powder and the hydrophobic chloroform-dissolved PLA. The HA nanopowder was dispersed effectively in HSA and mixed homogeneously with PLA. Continuous and uniform fibers were generated successfully with diameters of similar to 1-2 mu m, and featured a well-developed nanocomposite structure of HA nanopowder-dispersed PLA. Initial cellular assays showed excellent cell attachment and proliferation and also enhanced expression of alkaline phosphatase at 7 days of culturing. The HA-PLA nanocomposite fibers may be potentially useful in tissue engineering applications, particularly as three-dimensional substrates for bone growth. (c) 2006 Wiley Periodicals, Inc.