Contributions of low molecule number and chromosomal positioning to stochastic gene expression
Contributions of low molecule number and chromosomal positioning to stochastic gene expression
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DOI：
10.1038/ng1616
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发表时间：
2005-09-01
期刊：
影响因子：
30.8
通讯作者：
van Oudenaarden, A
中科院分区：
文献类型：
--
作者：
Becskei, A;Kaufmann, BB;van Oudenaarden, A
在调控网络中，低拷贝数调控和开关样信号传播的存在预计会增加细胞过程中的噪声。我们开发了一种噪声放大器，可以检测酵母中低丰度mrna水平的波动。观察到的波动不是由于基因本身表达的分子数量少，而是源于基因激活的随机、罕见事件。这些事件的频率和基因在单个细胞中随机表达之间的相关性取决于基因沿染色体的位置。由这种随机表达产生的转录调节因子将噪声传播到它们的靶基因。
The presence of low- copy- number regulators and switch- like signal propagation in regulatory networks are expected to increase noise in cellular processes. We developed a noise amplifier that detects fluctuations in the level of low- abundance mRNAs in yeast. The observed fluctuations are not due to the low number of molecules expressed from a gene per se but originate in the random, rare events of gene activation. The frequency of these events and the correlation between stochastic expressions of genes in a single cell depend on the positioning of the genes along the chromosomes. Transcriptional regulators produced by such random expression propagate noise to their target genes.