Understanding the molecular pathways associated with seed vigor.
Understanding the molecular pathways associated with seed vigor.
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DOI：
10.1016/j.plaphy.2012.07.031
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发表时间：
2012-11
期刊：
影响因子：
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通讯作者：
L. Ventura;M. Donà;A. Macovei;D. Carbonera;A. Buttafava;A. Mondoni;G. Rossi;A. Balestrazzi
中科院分区：
文献类型：
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作者：
L. Ventura;M. Donà;A. Macovei;D. Carbonera;A. Buttafava;A. Mondoni;G. Rossi;A. Balestrazzi
农民和种植者一直在寻找能够确保均匀田间建立和提高产量的高质量种子。种子引发可以诱导种子萌发前的代谢，从而提高种子萌发效率和作物产量。据推测，引发处理也可能提高发芽种子的胁迫耐受性，留下一种“胁迫记忆”。然而，引发的分子基础仍然需要澄清，鉴定种子活力的分子指标是当今种子生物学基础和应用研究的相关目标。普遍认为，增强的种子活力和成功的引发取决于在吸胀期间激活的DNA修复机制。DNA损伤控制/修复功能网络的复杂性在植物中仅得到部分阐明，并且涉及种子的具体文献仍然很少。此处描述的DNA修复途径（核苷酸和碱基切除修复、非同源末端连接、同源末端置换）发挥特定作用，所有这些对于确保基因组稳定性都是至关重要的。本文还重点介绍了DNA修复的一些新的调控机制（染色质重塑和小RNA），同时讨论了端粒序列作为种子库衰老标志物的可能用途。电子顺磁共振在阐明种子老化的动力学，在自由基配置文件和膜的完整性方面提供的显着贡献的报告。
Farmers and growers are constantly looking for high quality seeds able to ensure uniform field establishment and increased production. Seed priming is used to induce pre-germinative metabolism and then enhance germination efficiency and crop yields. It has been hypothesized that priming treatments might also improve stress tolerance in germinating seeds, leaving a sort of ‘stress memory’. However, the molecular bases of priming still need to be clarified and the identification of molecular indicators of seed vigor is nowadays a relevant goal for the basic and applied research in seed biology. It is generally acknowledged that enhanced seed vigor and successful priming depend on DNA repair mechanisms, activated during imbibition. The complexity of the networks of DNA damage control/repair functions has been only partially elucidated in plants and the specific literature that address seeds remains scanty. The DNA repair pathways hereby described (Nucleotide and Base Excision Repair, Non-Homologous End Joining, Homologous Recombination) play specific roles, all of them being critical to ensure genome stability. This review also focuses on some novel regulatory mechanisms of DNA repair (chromatin remodeling and small RNAs) while the possible use of telomere sequences as markers of aging in seed banks is discussed. The significant contribution provided by Electron Paramagnetic Resonance in elucidating the kinetics of seed aging, in terms of free radical profiles and membrane integrity is reported.