Induction of neovascularization and nonlymphoid mesenchymal cell proliferation by macrophage cell lines.
Induction of neovascularization and nonlymphoid mesenchymal cell proliferation by macrophage cell lines.
复制标题
巨噬细胞系诱导新血管形成和非淋巴间质细胞增殖。
DOI：
10.1002/jlb.37.3.279
复制
发表时间：
1985
影响因子：
5.5
通讯作者：
Leibovich,SJ
中科院分区：
文献类型：
--
作者：
Polverini,PJ;Leibovich,SJ
成熟的鼠类巨噬细胞样细胞 NCTC-3749 和 J-774、未成熟的人巨噬细胞样细胞 U-937-1 及其条件培养基在大鼠角膜中表现出有效的血管生成活性，并刺激牛主动脉内皮细胞 (BAEC) 的增殖和培养的 BALB/C-3T3 细胞中的 DNA 合成。相比之下，未成熟的人类巨噬细胞样细胞 HL-60 及其条件培养基未能产生或释放可检测到的这些活性。将 HL-60 细胞暴露于佛波醇肉豆蔻酸酯乙酸酯 (PMA) 并不会增强这些细胞的血管生成和生长刺激活性的表达。血管生成和生长刺激活性似乎都是由一种因子介导的，该因子具有与正常大鼠腹膜巨噬细胞产生的巨噬细胞衍生生长因子（MDGF）相同的生化特性。这些结果表明 NCTC-3749、J-774 和 U-937-1 巨噬细胞样细胞系可能是大规模生产和表征 MDGF 和巨噬细胞衍生的血管生成活性的有用来源。
The mature murine macrophage‐like cells NCTC‐3749 and J‐774, the Immature human macrophage‐like cells U‐937‐1, and their conditioned media exhibited potent angiogenic activity in rat corneas and stimulated proliferation of bovine aortic endothelial cells (BAEC) and DNA synthesis in BALB/C‐3T3 cells in culture. In contrast, the immature human macrophage‐like cells HL‐60 and their conditioned media either failed to produce or release detectable quantities of these activities. Exposure of HL‐60 cells to phorbol‐myristate‐acetate (PMA) did not enhance expression of angiogenic and growth stimulating activities by these cells. Both the angiogenic and growth stimulating activities appear to be mediated by a factor(s) that has biochemical properties in common with macrophage‐derived growth factor (MDGF) produced by normal rat peritoneal macrophages. These results suggest that NCTC‐3749, J‐774, and U‐937‐1 macrophage‐like cell lines may be a useful source for the large scale production and characterization of MDGF and macrophage‐derived angiogenic activity.