Function of antimicrobial proteins in insects.
Function of antimicrobial proteins in insects.
复制标题
昆虫抗菌蛋白的功能。
DOI：
10.1002/9780470514658.ch8
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发表时间：
1994
期刊：
影响因子：
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通讯作者：
Shunji Natori
中科院分区：
文献类型：
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作者：
Shunji Natori
我们从肉蝇幼虫血淋巴中分离并鉴定了多种抗微生物蛋白。其中，肉毒素I家族是一组主要对革兰氏阴性菌有活性的蛋白质，而沙培素主要对革兰氏阳性菌有活性。除了防御功能外，萨普菌素在昆虫发育中也扮演着重要角色。最近，我们发现了一种与原皂素B具有相同抗菌活性的皂素同源物皂素B的多肽。肉毒毒素I和皂素都是昆虫对细菌感染处于急性期时由脂肪体和/或血细胞重新合成并分泌到血淋巴中的诱导蛋白。抗真菌蛋白(AFP)存在于血淋巴中，对某些真菌有效，但对细菌无效。这些不同的抗菌蛋白与微生物膜相互作用。肉毒素I干扰膜功能，如三磷酸腺苷合成和氨基酸转运。AFP的杀菌活性可被肉毒毒素I协同增强，尽管单独的肉毒毒素I没有明显的抗真菌活性。很明显，果蝇有能力通过动员几种抗微生物蛋白来对微生物寄生虫产生强大的防御反应。
We have isolated and characterized various antimicrobial proteins from the haemolymph of Sarcophaga peregrina (flesh fly) larvae. Of these the sarcotoxin I family is a group of proteins mainly active against Gram-negative bacteria whereas sapecin is active mainly against Gram-positive bacteria. In addition to its function in defence, sapecin also plays a role in insect development. Recently, we identified a hendecapeptide of the sapecin homologue sapecin B that has the same antibacterial activity as the original sapecin B. Both sarcotoxin I and sapecin are inducible proteins synthesized de novo by the fat body and/or haemocytes and secreted into the haemolymph when the insect is in the acute phase response to bacterial infection. Antifungal protein (AFP) is constitutively present in the haemolymph and is active against certain fungi but not bacteria. These various antimicrobial proteins interact with microbial membranes. Sarcotoxin I interferes with membrane functions such as ATP synthesis and amino acid transport. The fungicidal activity of AFP is enhanced synergistically by sarcotoxin I, although sarcotoxin I alone has no appreciable antifungal activity. It is clear that the flesh fly has the ability to mount a potent defence response against microbial parasites by mobilizing several antimicrobial proteins.
影响因子：
3.1
作者：
BOMAN, HG;AGERBERTH, B;BOMAN, A
通讯作者：
BOMAN, A
Kenji Matsuyama 和 Shunji Natori：“从 NIH-Sape-4（Sarcophaga peregrina 胚胎细胞系）培养基中纯化三种抗菌蛋白”J. Biol。
DOI：
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