A conditional yeast mutant deficient in mRNA transport from nucleus to cytoplasm.
A conditional yeast mutant deficient in mRNA transport from nucleus to cytoplasm.
复制标题
一种条件性酵母突变体，其 mRNA 从细胞核转运至细胞质的能力存在缺陷。
DOI：
10.1073/pnas.89.6.2312
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发表时间：
1992
影响因子：
11.1
通讯作者：
Tartakoff,AM
中科院分区：
文献类型：
--
作者：
Kadowaki,T;Zhao,Y;Tartakoff,AM
mRNA 从细胞核到细胞质的转运对于真核基因表达至关重要；然而，出口机制尚不清楚。因此，已使用选择和筛选程序来获得酿酒酵母的温度敏感条件突变体家族，根据原位杂交判断，当在 37°C 温育时，这些突变体在细胞核中积累聚（A）+ RNA。在一种这样的mRNA转运突变体mtr1-1中，RNA合成继续进行，poly(A)+ RNA的输出受到抑制，核内poly(A)+非常稳定，并且蛋白质合成逐渐停止。因此，RNA 合成和输出之间不存在紧密耦合。出口损伤是可逆的。虽然 mRNA 输出显然不是默认选项，但根据原位杂交判断，无论是抑制蛋白质合成、抑制 mRNA 剪接还是抑制 Poly(A) 结合蛋白功能，都不会阻止平均 Poly(A)+ 的输出。对 mtr 突变体家族的进一步分析应该有助于绘制 RNA 运输路径。
Transport of mRNA from nucleus to cytoplasm is critical for eukaryotic gene expression; however, the mechanism of export is unknown. Selection and screening procedures have therefore been used to obtain a family of temperature-sensitive conditional mutants of Saccharomyces cerevisiae that accumulate poly(A)+ RNA in the nucleus when incubated at 37 degrees C, as judged by in situ hybridization. In one such mRNA transport mutant, mtr1-1, RNA synthesis continues, the export of poly(A)+ RNA is inhibited, intranuclear poly(A)+ is remarkably stable, and protein synthesis gradually stops. Thus, there is no tight coupling between RNA synthesis and export. The export lesion is reversible. Although mRNA export is clearly not a default option, neither inhibition of protein synthesis, inhibition of mRNA splicing, nor inhibition of poly(A)-binding protein function blocks export of the average poly(A)+, as judged by in situ hybridization. Further analysis of the family of mtr mutants should help map the path of RNA transport.