Shi Xiao San ameliorates the development of adenomyosis in an ICR mouse model.
Shi Xiao San ameliorates the development of adenomyosis in an ICR mouse model.
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DOI：
10.3892/etm.2020.8994
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发表时间：
2020-07
影响因子：
2.7
通讯作者：
Juan Ye;Xu-jun Cai;Dawei Wang;Furong Zhang;Zhi-gang Wang;M. Cao;Zhonghua Pang;Jie Yang
中科院分区：
文献类型：
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作者：
Juan Ye;Xu-jun Cai;Dawei Wang;Furong Zhang;Zhi-gang Wang;M. Cao;Zhonghua Pang;Jie Yang
由蒲黄、五灵脂组成的石消散的使用可追溯到宋代。传统上，SXS已被用于治疗月经不调，盆腔疼痛，进行性痛经，产后恶露不绝。子宫腺肌病（AM）的管理是具有挑战性的，据我们所知，目前还没有有效的治疗策略。因此，本研究的目的是研究SXS对小鼠子宫腺肌病发展的影响。采用三苯氧胺诱导60只ICR小鼠AM模型，随机抽取10只小鼠进行病理组织学检查，另10只小鼠作为正常对照。生后60 d，将AM处理的小鼠随机分为4组，分别给予SXS低剂量组（55 mg/kg）、SXS高剂量组（110 mg/kg）、达那唑组（1 mg/20 g体重）和模型组（不处理），同时设正常对照组。治疗2个月后，使用热板和尾部甩尾试验评估小鼠对有害热刺激的反应，并收集血浆样品以测量皮质酮水平。苏木精-伊红染色观察肌层浸润情况及AM结节数。此外，还分析了与AM相关疼痛相关的基因的表达。本研究结果表明，SXS治疗减少子宫肌层浸润，减轻全身性痛觉过敏，并降低血浆皮质酮水平诱导AM小鼠。这些结果表明，SXS有效地减弱了AM的发展，并可能作为AM治疗的一种有前途的治疗方法。
The use of Shi Xiao San (SXS), composed of Pollen Typhae Angustifoliae and Faeces Trogopterori, can be traced back to the Song dynasty. Traditionally, SXS has been used to treat irregular menstruation, pelvic pain, progressive dysmenorrhea, and postpartum lochiorrhea. The management of adenomyosis (AM) is challenging and to the best of our knowledge there are currently no effective therapeutic strategies. Therefore, the aim of the present study was to investigate the effect of SXS on the development of adenomyosis in a mouse model. AM was induced in 60 neonatal female ICR mice by administering tamoxifen; 10 randomly selected mice were used for model identification via histopathological examination and 10 mice treated with the solvent alone were used as the normal controls. A total of sixty days after birth, the mice treated with AM were randomly divided into four groups and administered one of the following treatments: Low-dose SXS (55 mg/kg); high-dose SXS (110 mg/kg); danazol (1 mg/20 g body weight); or no treatment (model group); at the same time, the normal control group received no treatment. After 2 months of treatment, hotplate and tail-flick tests were used to assess the response to noxious thermal stimuli in the mice, and plasma samples were collected to measure corticosterone levels. Hematoxylin and eosin staining scores of myometrial infiltration and the number of AM nodules were evaluated. Furthermore, the expression of genes associated with AM-related pain was also analyzed. The results from the present study indicated that treatment with SXS decreased myometrial infiltration, alleviated generalized hyperalgesia, and lowered plasma corticosterone levels in mice with induced AM. These findings suggest that SXS effectively attenuated the development of AM, and may serve as a promising treatment approach for AM treatment.