Effects of atorvastatin in the regulation of circulating EPCs and angiogenesis in traumatic brain injury in rats
Effects of atorvastatin in the regulation of circulating EPCs and angiogenesis in traumatic brain injury in rats
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DOI：
10.1016/j.jns.2012.04.015
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发表时间：
2012-08-15
影响因子：
4.4
通讯作者：
Jiang, Rongcai
中科院分区：
文献类型：
--
作者：
Wang, Bin;Sun, Lin;Jiang, Rongcai
循环内皮祖细胞（EPC）在血管生成和血管发生中发挥重要作用。他汀类药物可促进大鼠的功能改善，而与降低胆固醇的能力无关。尚未研究他汀类药物治疗是否能调节创伤性脑损伤 (TBI) 后的循环 EPC。我们假设阿托伐他汀会增加 TBI 大鼠的循环 EPC 并促进血管生成。 Wistar 大鼠（20 个月大）接受 TBI，并在 TBI 后 1 小时开始用或不用阿托伐他汀（口服给药，1 mg/kg/天）治疗，然后每天治疗，连续 14 天。进行了海马角的长时程增强 (LTP) 以及改良神经严重程度评分 (mNSS) 和莫里斯水迷宫 (MWM) 功能测试。通过流式细胞术鉴定血液循环EPC。 TBI后25天处死大鼠。进行 vWF 和 CD31 免疫染色。我们发现，与未治疗的 TBI 对照大鼠相比，阿托伐他汀给药显着诱导血管生成、增加循环 EPC 水平以及改善功能恢复 (P
Circulating endothelial progenitor cells (EPCs) play an important role in angiogenesis and vasculogenesis. Statins administered promote functional improvement in rats, independent of their capability to lower cholesterol. Whether statin treatment regulates circulating EPCs after traumatic brain injury (TBI) has not been investigated. We hypothesized that atorvastatin increases circulating EPCs and promotes angiogenesis in TBI rats. Wistar rats (20 months old) were subjected to TBI and treated with or without atorvastatin (orally administered, 1 mg/kg/day) starting 1 h after TBI and then daily for 14 consecutive days. Long term potentiation (LTP) in the cornu ammonis1 of the hippocampus as well as the Modified Neurological Severity Score (mNSS) and the Morris Water Maze (MWM) functional tests were performed. Blood circulating EPCs were identified by flow cytometry. Rats were sacrificed 25 days after TBI. vWF and CD31 immunostaining was performed. We found that atorvastatin administration significantly induced angiogenesis and increased circulating EPC levels as well as improved functional recovery when compared with non-treatment TBI-control rats (P