Nuclear endogenous Ca2+‐dependent endodeoxyribonuclease in differentiating chick embryonic lens fibers
Nuclear endogenous Ca2+‐dependent endodeoxyribonuclease in differentiating chick embryonic lens fibers
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核内源性Ca2+依赖性内脱氧核糖核酸酶在分化鸡胚晶状体纤维中的作用
DOI：
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发表时间：
1986
影响因子：
5.6
通讯作者：
M. Counis
中科院分区：
文献类型：
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作者：
A. S. Muel;E. Chaudun;Y. Courtois;S. Modak;M. Counis
在透镜上皮细胞终末分化为纤维细胞的过程中，细胞核变致密，DNA发生降解。我们研究了内源性DNA酶在这一过程中的假定作用。在37°C下孵育两种细胞类型的分离细胞核后，通过在0.3-1%中性和碱性琼脂糖、一维和二维凝胶上进行DNA大小分析来揭示DNA酶活性。这种DNA酶活性是更突出的透镜纤维核比上皮细胞核在所有的胚胎阶段，可能是因为预先存在的二价阳离子的浓度较高的前者。这种活性在两种类型的细胞核中都依赖于钙或镁。
During terminal differentiation of lens epithelial cells into fiber cells, nuclei become pycnotic and DNA degradation occurs. We investigated the putative role in this process of an endogenous DNAase. After incubation of isolated nuclei of both cell types at 37°C, DNAase activity was revealed by DNA size analysis on 0.3–1% neutral and alkaline agarose, one‐ and two‐dimensional gels. This DNAase activity is more prominent in lens fiber nuclei than in epithelial nuclei at all the embryonic stages probably because of a preexisting higher concentration of divalent cations in the former. This activity is calcium or magnesium dependent in both types of nuclei.