Direct effects of manganese compounds on dopamine and its metabolite Dopac: an in vitro study.
Direct effects of manganese compounds on dopamine and its metabolite Dopac: an in vitro study.
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锰化合物对多巴胺及其代谢物 Dopac 的直接影响：一项体外研究。
DOI：
10.1016/j.etap.2006.11.004
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发表时间：
2007
影响因子：
4.3
通讯作者：
Filipov,NikolayM
中科院分区：
文献类型：
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作者：
Sistrunk,ShannonC;Ross,MatthewK;Filipov,NikolayM
在含有添加剂甲基环戊二烯基-三羰基锰（MMT）的燃料燃烧后，磷酸锰（MnPO 4）和硫酸锰（MnSO 4）被排放到大气中。氯化锰（MnCl 2），另一种Mn 2+物质，在实验中被广泛使用。使用大鼠纹状体切片，我们发现，MnPO 4降低组织和介质多巴胺（DA）和介质Dopac（DA代谢物）水平大大超过MnCl 2或MnSO 4;抗氧化剂是部分保护。此外，MnCl 2和MnPO 4（更有力）氧化DA和多巴，即使在介质中的组织的情况下，表明Mn和DA/多巴之间的直接相互作用。由于氨基色素是DA的主要氧化产物，我们接下来确定MnPO 4是否比MnCl 2或MnSO 4更有效地形成氨基色素，事实确实如此。因此，一个潜在的额外机制的神经毒性作用的环境相关形式的锰，特别是MnPO 4，是反应性DA中间体的产生。
Following combustion of fuel containing the additive methylcyclopentadienyl-manganese-tricarbonyl (MMT), manganese phosphate (MnPO4) and manganese sulfate (MnSO4) are emitted in the atmosphere. Manganese chloride (MnCl2), another Mn2+species, is widely used experimentally. Using rat striatal slices, we found that MnPO4decreased tissue and media dopamine (DA) and media Dopac (a DA metabolite) levels substantially more than either MnCl2or MnSO4; antioxidants were partially protective. Also, both MnCl2and MnPO4(more potently) oxidized DA and Dopac even in the absence of tissue in the media, suggesting a direct interaction between Mn and DA/Dopac. Because aminochrome is a major oxidation product of DA, we next determined whether MnPO4will be more potent in forming aminochrome than MnCl2or MnSO4which, indeed, was the case. Thus, a potential additional mechanism for the neurotoxic effects of environmentally relevant forms of Mn, MnPO4in particular, is the generation of reactive DA intermediates.