PITUITARY EXTRACTS AND STEROID-HORMONES IN CONTROL 3T3-CELL GROWTH
PITUITARY EXTRACTS AND STEROID-HORMONES IN CONTROL 3T3-CELL GROWTH
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DOI：
10.1073/pnas.70.9.2702
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发表时间：
1973-01-01
影响因子：
11.1
通讯作者：
ARMELIN, HA
中科院分区：
文献类型：
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作者：
ARMELIN, HA
结果显示垂体提取物刺激3 T3细胞的生长，3 T3细胞是一种已建立的小鼠成纤维细胞系。在含有有机缓冲液（HEPES;N-2-羟乙基哌嗪-N ′-2-乙磺酸）的培养基中培养细胞，该缓冲液可增加细胞生长的血清需求。垂体促生长活性是（a）不可透析的;（B）不耐热的;（c）蛋白酶敏感的;和（d）被氢化可的松（类固醇激素）增强的。通过测量细胞分裂刺激或[3 H]胸苷摄取到DNA中来定量测定活性。活性物质可能与垂体蛋白激素不同，在体内观察到的蛋白酶浓度下似乎有效。由猿猴病毒40转化的3 T3细胞对这种活性没有反应。
Results are reported showing that pituitary extracts stimulate growth of 3T3 cells, an established line of mouse fibroblast. Cells were cultured in medium containing an organic buffer (HEPES;N-2-hydroxyethylpiperazine-N′-2-ethanesulfonic acid), which increases the serum requirement for cell growth. The pituitary growth-prompting activity is (a) not dialyzable; (b) thermolabile; (c) protease sensitive; and (d) enhanced by hydrocortisone (a steroid hormone). Activity was quantitatively assayed by measurement of stimulation of cell division or [3H]thymidine uptake into DNA. The active species is probably different from pituitary protein hormones and it seems to be effective at concentrations observed for protein hormonesin vivo. 3T3 cells transformed by simian virus 40 are not responsive to this activity.