CHARACTERIZATION OF MYOSIN ISOFORMS IN SATELLITE CELL-CULTURES FROM ADULT-RAT DIAPHRAGM, SOLEUS AND TIBIALIS ANTERIOR MUSCLES
CHARACTERIZATION OF MYOSIN ISOFORMS IN SATELLITE CELL-CULTURES FROM ADULT-RAT DIAPHRAGM, SOLEUS AND TIBIALIS ANTERIOR MUSCLES
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DOI：
10.1111/j.1432-0436.1990.tb00472.x
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发表时间：
1990-12-01
期刊：
影响因子：
2.9
通讯作者：
PETTE, D
中科院分区：
文献类型：
--
作者：
DUSTERHOFT, S;YABLONKAREUVENI, Z;PETTE, D
用酶解分离和Percoll梯度离心法从成年大鼠的横隔肌、比目鱼肌和胫前肌中分离出卫星细胞，获得了重复性较好的细胞。横隔肌和比目鱼肌产生的卫星细胞大约是胫骨前肌的五倍。根据光镜观察标准，不同来源的卫星细胞培养物在形态上没有差异。与供体肌肉相反，来自培养10天的卫星细胞的肌管含有统一的肌球蛋白重链(MHC)模式，其优势是免疫化学鉴定的胚胎重链。这三种类型的培养物呈现典型的胚胎轻链(LC)模式，有LC_1emb、LC_1f、LC_2f和LC_3f痕迹。异肌球蛋白由四种胚胎肌球蛋白EM1-eM4组成，在三种培养物中的分布相似。总之，这些肌球蛋白分析没有提供证据表明，至少在体外应用的条件下，不同纤维类型组成的三块大鼠肌肉之间存在卫星细胞多样性。
Satellite cells were isolated by enzymatic dissociation and Percoll gradient centrifugation from adult rat diaphragm, soleus, and tibialis anterior muscles with fairly reproducible yields. Diaphragm and soleus muscle yielded approximately five times more satellite cells than tibialis anterior muscle. According to light microscopic criteria, no morphological differences existed between the satellite cell cultures of different origin. Contrary to the donor muscles, myotubes from the 10-day-cultured satellite cells contained a uniform myosin heavy chain (MHC) pattern with predominance of an immunochemically identified embryonic heavy chain. The three types of cultures displayed a typical embryonic light chain (LC) pattern with LC1emb, LC1f, LC2f, and traces of LC3f. The isomyosin pattern was characterized by four embryonic isomyosins, eM1-eM4, with similar distributions in the three cultures. In summary, these myosin analyses provide no evidence for the existence of satellite cell diversity among three rat muscles of different fiber-type composition, at least not under the applied in vitro conditions.