Proteomic analysis of salicylic acid-induced resistance to Magnaporthe oryzae in susceptible and resistant rice
Proteomic analysis of salicylic acid-induced resistance to Magnaporthe oryzae in susceptible and resistant rice
复制标题
水杨酸诱导的敏感和抗性水稻对稻瘟病菌的抗性的蛋白质组学分析
DOI：
10.1002/pmic.201200054
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发表时间：
2012-08-01
期刊：
影响因子：
3.4
通讯作者：
Wang, Zhenzhong
中科院分区：
文献类型：
--
作者：
Li, Yunfeng;Zhang, Zhihui;Wang, Zhenzhong
为探讨水杨酸（SA）诱导的水稻稻瘟病菌（Magnaporthe Magnaporthe）转译水平上的序列事件及其相关因素，对SA处理后不同时间点的水稻抗、感品种进行了毒力测定和蛋白质组学分析。结果表明，水杨酸显著增强了水稻对稻瘟病菌的抗性。米。SA处理叶片的蛋白质组学分析揭示了36个差异表达的蛋白质，涉及各种功能，包括防御，抗氧化酶和信号转导。除了三种抗氧化酶被SA负调控外，大多数蛋白质都被诱导。与上述研究结果一致，SA增加活性氧（ROS）的水平与抗性品种C101 LAC表现出更快的响应SA和产生更高水平的ROS比敏感品种CO39。此外，我们发现，核苷二磷酸激酶1，这是在ROS的生产调节牵连，强烈诱导C101 LAC，但不是在CO39。这些结果表明，抗病品种可能具有比感病品种更敏感的SA信号系统或有效途径。此外，我们的研究结果表明，SA还协调其他细胞活动，如光合作用和代谢，以促进防御反应和恢复，突出了SA诱导的抗性机制的复杂性。
To probe salicylic acid (SA)-induced sequential events at translational level and factors associated with SA response, we conducted virulence assays and proteomic profiling analysis on rice resistant and susceptible cultivars against Magnaporthe oryzae at various time points after SA treatment. The results showed that SA significantly enhanced rice resistance against M. oryzae. Proteomic analysis of SA-treated leaves unveiled 36 differentially expressed proteins implicated in various functions, including defense, antioxidative enzymes, and signal transduction. Majority of these proteins were induced except three antioxidative enzymes, which were negatively regulated by SA. Consistent with the above findings, SA increased the level of reactive oxygen species (ROS) with resistant cultivar C101LAC showing faster response to SA and producing higher level of ROS than susceptible cultivar CO39. Furthermore, we showed that nucleoside diphosphate kinase 1, which is implicated in regulation of ROS production, was strongly induced in C101LAC but not in CO39. Taken together, the findings suggest that resistant rice cultivar might possess a more sensitive SA signaling system or effective pathway than susceptible cultivar. In addition, our results indicate that SA also coordinates other cellular activities such as photosynthesis and metabolism to facilitate defense response and recovery, highlighting the complexity of SA-induced resistance mechanisms.