Transcriptome analysis of Sinorhizobium meliloti during symbiosis.
Transcriptome analysis of Sinorhizobium meliloti during symbiosis.
复制标题
在共生期间米洛洛氏菌的转录组分析。
DOI：
10.1186/gb-2003-4-2-r15
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发表时间：
2003
期刊：
影响因子：
12.3
通讯作者：
Batut, Jacques
中科院分区：
文献类型：
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作者：
Ampe, Frederic;Kiss, Erno;Sabourdy, Frederique;Batut, Jacques
最近确定的苜蓿中华根瘤菌基因组序列被用来构建尼龙宏阵列，以寻找与共生潜在相关的基因。与入侵、氧化应激保护、铁动员和信号传导相关的基因被发现强调了共生和发病机制之间可能存在的共同机制。根瘤菌诱导特定豆科植物新器官（根瘤）的形成，这是两个合作伙伴精心设计的发育计划的结果。为了更全面地了解这种植物-微生物共生背后的遗传学，我们挖掘了最近确定的苜蓿中华根瘤菌基因组序列，以寻找可能与共生相关的基因。我们在这里描述了专用尼龙宏阵列的构建和使用，以同时研究 200 个这些基因在与共生相关的各种环境条件下的表达。在十种环境条件下监测了214个苜蓿基因的表达，包括自由生活的有氧和微需氧条件、植物共生诱导子木犀草素的添加以及各种共生条件。已鉴定出木犀草素诱导的 5 个新基因以及成熟固氮类杆菌中诱导的 9 个新基因。已发现在根瘤发育中受影响的细菌和植物共生突变体对于破译共生相互作用的中间阶段的基因表达特别感兴趣。对栽培豆科植物紫花苜蓿（Medicago sativa）和模式植物蒺藜苜蓿（M. truncatula）中苜蓿基因表达进行比较，发现少量差异。除了探索模型根瘤菌全基因组转录组分析的条件外，目前的工作还强调了共生期间几类基因的差异表达。这些基因与入侵、氧化应激保护、铁动员和信号传导有关，从而强调了共生和发病机制之间可能存在的共同机制。
The recently determined Sinorhizobium meliloti genome sequence was used to construct nylon macroarrays, to search for genes potentially relevant to symbiosis. Genes related to invasion, oxidative stress protection, iron mobilization, and signaling, were found emphasizing possible common mechanisms between symbiosis and pathogenesis. Rhizobia induce the formation on specific legumes of new organs, the root nodules, as a result of an elaborated developmental program involving the two partners. In order to contribute to a more global view of the genetics underlying this plant-microbe symbiosis, we have mined the recently determined Sinorhizobium meliloti genome sequence for genes potentially relevant to symbiosis. We describe here the construction and use of dedicated nylon macroarrays to study simultaneously the expression of 200 of these genes in a variety of environmental conditions, pertinent to symbiosis. The expression of 214 S. meliloti genes was monitored under ten environmental conditions, including free-living aerobic and microaerobic conditions, addition of the plant symbiotic elicitor luteolin, and a variety of symbiotic conditions. Five new genes induced by luteolin have been identified as well as nine new genes induced in mature nitrogen-fixing bacteroids. A bacterial and a plant symbiotic mutant affected in nodule development have been found of particular interest to decipher gene expression at the intermediate stage of the symbiotic interaction. S. meliloti gene expression in the cultivated legume Medicago sativa (alfalfa) and the model plant M. truncatula were compared and a small number of differences was found. In addition to exploring conditions for a genome-wide transcriptome analysis of the model rhizobium S. meliloti, the present work has highlighted the differential expression of several classes of genes during symbiosis. These genes are related to invasion, oxidative stress protection, iron mobilization, and signaling, thus emphasizing possible common mechanisms between symbiosis and pathogenesis.