Towards mosquito sterile insect technique programmes: Exploring genetic, molecular, mechanical and behavioural methods of sex separation in mosquitoes
Towards mosquito sterile insect technique programmes: Exploring genetic, molecular, mechanical and behavioural methods of sex separation in mosquitoes
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DOI：
10.1016/j.actatropica.2013.08.015
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发表时间：
2014-04-01
期刊：
影响因子：
2.7
通讯作者：
Bourtzis, Kostas
中科院分区：
文献类型：
--
作者：
Gilles, Jeremie R. L.;Schetelig, Marc F.;Bourtzis, Kostas
在考虑蚊子释放计划时，首先要解决的问题之一是如何消灭/隔离雌性蚊子。对于那些可以使用转基因蚊子的人来说，最终可能会有最多的选择，但目标物种的固有特征也可能为临时措施提供可能性，直到开发出更有效的方法。雌性和雄性在内在大小、行为和发育速度上的差异通常可以用来进行性别鉴定。已经开发出有效的针对特定物种的系统，用于在胚胎阶段淘汰雌性动物，但由于缺乏使用，大多数已经被丢弃。理想的系统专门使用一些可以在生产过程中操纵的处理方法来杀死女性胚胎。这样的杀戮系统比使用固有的性别差异要有效得多，但它们的系统需要可选择的遗传标记和由罕见的随机染色体重排产生的性别连锁。虽然不应将固有的性别差异视为发展强有力和有效的性别鉴定办法的长期备选办法，但在缺乏这些办法的情况下，可利用和整合效率较低的系统可以提供一种权宜之计，允许以最少的投资快速启动。国际原子能机构正在资助一个新的协调研究项目，该项目将在5年内(2013-2018年)探索蚊子性别分离的遗传、分子、机械和行为方法，以建立研究人员网络，并解决实施昆虫不育技术(使用辐射不育或转基因雄性蚊子)和针对传播疾病的蚊子的昆虫不相容技术方案的遗传性别鉴定菌株的关键需求。版权所有(C)国际原子能机构2013。爱思唯尔出版，版权所有。
hen considering a mosquito release programme, one of the first issues to be addressed is how to eliminate/separate the females. The greatest number of options might eventually be available for those who can use transgenic mosquitoes, but the inherent characteristics of the target species may also provide possibilities for interim measures until more efficient methods can be developed. Differences in intrinsic size, in behaviour and in development rate between females and males are often available and useful for sexing. Efficient species-specific systems for eliminating females at the embryo stage have been developed, but most have since been discarded due to lack of use. Ideal systems specifically kill female embryos using some treatment that can be manipulated during production. Such killing systems are far more efficient than using intrinsic sexual differences, but they systems require selectable genetic markers and sex-linkage created by rare random chromosomal rearrangements. While intrinsic sexual differences should not be considered as long-term candidates for the development of robust and efficient sexing approaches, in the absence of these, the accessibility and integration of less efficient systems can provide a stop-gap measure that allows rapid start up with a minimum of investment. The International Atomic Energy Agency is funding over a 5 year period (2013-2018) a new Coordinated Research Project on "Exploring Genetic, Molecular, Mechanical and Behavioural Methods of Sex Separation in Mosquitoes" to network researchers and to address the critical need of genetic sexing strains for the implementation of the sterile insect technique (using radiation-sterilised or transgenic male mosquitoes) and for insect incompatibility technique programmes against disease-transmitting mosquitoes. Copyright (C) International Atomic Energy Agency 2013. Published by Elsevier B.V. All rights reserved.