Diphenylamine-aniline-phosphoric acid reagent, a versatile spray reagent for revealing glycoconjugates on thin-layer chromatography plates
Diphenylamine-aniline-phosphoric acid reagent, a versatile spray reagent for revealing glycoconjugates on thin-layer chromatography plates
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DOI：
10.1006/abio.2000.4829
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发表时间：
2000-12-15
影响因子：
2.9
通讯作者：
Li, YT
中科院分区：
文献类型：
--
作者：
Anderson, K;Li, SC;Li, YT
尽管有许多喷雾试剂可用于在薄层色谱（TLC）2板上显示糖缀合物，但很少有喷雾试剂能够显示糖缀合物中发现的所有类型的单糖。例如，用于在TLC板上显示糖缀合物的两种最常用的喷雾试剂是用于含中性糖的糖缀合物的Orcinol-H2SO 4试剂（Orcinol）（1，2）和用于含唾液酸的1的间苯二酚-HCl试剂（间苯二酚）（3）。传真：（504）584-2739。电子邮件：yli@ tmcpop。tmc。杜兰edu. 2所用缩略语：TLC，薄层色谱法; DPA，二苯胺-苯胺-磷酸; KDO，3-脱氧-D-甘露-辛酮糖酸; KDN，2-酮基-3-脱氧-D-甘油-D-半乳糖酸，与3-脱氧-D-甘油-D-半乳糖酸和脱氨基神经氨酸同义。
Although numerous spray reagents are available for revealing glycoconjugates on thin-layer chromatography (TLC) 2 plates, few are able to reveal all types of monosaccharides found in glycoconjugates. For example, two of the most commonly used spray reagents for revealing glycoconjugates on TLC plates are the orcinol–H2SO4 reagent (Orcinol)(1, 2) for neutral sugarcontaining glycoconjugates and the resorcinol–HCl reagent (Resorcinol)(3) for sialic acid-containing1 To whom correspondence should be addressed. Fax:(504) 584-2739. E-mail: yli@ tmcpop. tmc. tulane. edu. 2 Abbreviations used: TLC, thin-layer chromatography; DPA, diphenylamine–aniline–phosphoric acid; KDO, 3-deoxy-D-manno-octulosonic acid; KDN, 2-keto-3-deoxy-D-glycero-D-galactonononic acid, which is synonymous with 3-deoxy-D-glycero-D-galactononulosonic acid and deaminated neuraminic acid.