Antiprotozoal and cytotoxic screening of 45 plant extracts from Democratic Republic of Congo
Antiprotozoal and cytotoxic screening of 45 plant extracts from Democratic Republic of Congo
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DOI：
10.1016/j.jep.2007.10.028
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发表时间：
2008-02-12
影响因子：
5.4
通讯作者：
Vlietinck, A. J.
中科院分区：
文献类型：
--
作者：
Mesia, G. K.;Tona, G. L.;Vlietinck, A. J.
研究目的：对采自桑库鲁的45种药用植物的80%甲醇提取物进行体外抗原虫和细胞毒活性评价（刚果民主共和国）分别针对布氏锥虫、克氏锥虫和恶性疟原虫的氯喹敏感性加纳菌株以及MRC-5细胞系进行的试验。各部位用80%甲醇浸渍24 h，得到不同的提取物。将混合物过滤并真空蒸发，得到相应的干燥提取物。抗布氏锥虫和克氏锥虫的活性在96孔组织板中进行，每个板含有10 RI水性植物提取物稀释液（100 - 0.01 μ g/ml）与10 μ l在补充有10%胎牛血清、2%青霉素/链霉素（2%P/S）溶液的Hirumi培养基中培养的寄生虫悬浮液一起培养。在500 nm发射和530 nm激发下测量荧光，结果表示为与对照威尔斯孔相比寄生虫减少的百分比。抗疟原虫活性进行了评估，在体外对氯喹敏感的恶性疟原虫加纳株在RPMI-1640培养基中培养的乳酸脱氢酶测定在植物提取物（50至0.01 μ g/ml）的存在下。在补充有20 mM L-谷氨酰胺、16.5 mM NaHCO 3、5%胎牛血清和2% P/S溶液的MEM培养基中培养细胞系MRC-5。孵育4小时后，加入MTT后，在540 nm处分光光度法评估细胞增殖/活力。结果：苦马豆叶、苦瓜全株、蒲桃、根皮和非洲黄皮藤茎皮的提取物对布氏锥虫具有较好的抗原虫活性，IC_（50）值为0.7 ~ 7 μ g/ml。只有非洲瓶霉提取物显示出明显的抗克氏锥虫的抗原动物活性（IC 50 = 4.0 +/- 06 μ g/ml）。从Alchornea cordifolia，Polyathia swaveletum茎皮，Sapium cornutum茎皮和Triclisia giletii茎皮的提取物对恶性疟原虫加纳株表现出明显的抗疟原虫活性，IC 50值为0.5 - 3.0 μ g/ml。对所有选择的原生动物（SI < 10），而其他活性提取物对MCR-5细胞系没有显示出明显的细胞毒性作用，根据情况具有良好的选择性。结论：这些活性植物提取物被选择用于广泛的研究，导致活性成分的分离。(c)2007爱思唯尔爱尔兰有限公司保留所有权利。
Aim of the study: To evaluate in vitro the antiprotozoal and cytotoxic activies of 80% methanol extract from 45 medicinal plants collected in Sankuru (Democratic Republic of Congo) against Trypanosoma brucei brucei, Trypanosoma cruzi and the chloroquine-sensitive Ghanaian strain of Plasmodium falciparum, and MRC-5 cell lines respectively.Material and methods: Differents extracts were obtained by maceration of each plant part used with 80% methanol for 24 h. The mixture was filtered and evaporated in vacuo to give corresponding dried extract. The activity against Trypanosoma brucei brucei and Trypanosoma cruzi were performed in 96 well tissue plates each containing 10 RI aqueous plant extract dilutions (100 to 0.01 mu g/ml) with 10 mu l of the parasite suspension cultured in Hirumi medium supplemented with 10% foetal calf serum, a solution of 2% penicillin/streptomycin (2% P/S) After 4 days incubation with Almar bluea solution, fluorescence was measured at 500 nm emission and 530 nm excitation and results expressed as percentage reduction in parasite compared to control wells. The antiplasmodial activity of was assessed in vitro against the chloroquine-sensitive Ghanaian strain of Plasmodium falciparum cultured in RPMI-1640 medium by the lactate deshydrogenase assay in the presence of plant extracts (50 to 0.01 mu g/ml). Cell-lines MRC-5 were cultured in MEM medium supplemented with 20 MM L-glutamine, 16.5 mM NaHCO3, 5% foetal calf serum and 2% P/S solution. After 4 h incubation, cell proliferation/viability was spectrophotornecally assessed at 540 nm after addition of MTT. In each assay, the IC50 value for each sample was derived by the drug concentration-response curves.Results: The extracts from Alcornea cordifolia leaves, Momordica charantia whole plant, Omphalocarpum glomerata, root bark and Piptadia africanum stem bark showed good antiprotozoal activity against Trypanosoma brucei brucei with IC50 values from 0.7 to 7 mu g/ml. Only Piptadenia africanum extract showed a pronounced antiprotozoal activity against Trypanosoma cruzi (IC50 = 4.0 +/- 06 mu g/ml). The extracts from Alchornea cordifolia, Polyathia swaveleons stem bark, Sapium cornutum stem bark and Triclisia giletii stem bark exhibited a pronounced antiplasmodial activity against P. falciparum Ghanaian strain with IC50 values ranging from 0.5 to 3.0 mu g/ml. Piptadenia africanum extract was the most cytotoxic sample (CC50 = 0.25 mu g/ml) with poor selectivity against all selected protozoa (SI < 10) while other active extracts did not show a significant cytotoxic effect against MCR-5 cell-lines with good selectivity according to the case.Conclusion: These active plant extracts are selected for extensive studies leading to the isolation of active constituents. (c) 2007 Elsevier Ireland Ltd. All rights reserved.