The clustering of four subfamilies of satellite DNA at individual chromosome ends in Silene latifolia
The clustering of four subfamilies of satellite DNA at individual chromosome ends in Silene latifolia
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DOI：
10.1139/g05-130
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发表时间：
2006-05-01
期刊：
影响因子：
3.1
通讯作者：
Kawano, Shigeyuki
中科院分区：
文献类型：
--
作者：
Kazama, Yusuke;Sugiyama, Ryuji;Kawano, Shigeyuki
从雌雄异株植物宽叶蝇子草中分离到染色体末端的卫星DNA。分离在串联阵列中含有大量satDNA重复单元的BAC克隆以检查重复单元的聚类。从每个分离的BAC克隆中纯化satDNA重复单元并测序。为了研究重复单元之间的成对相似性，使用邻接算法构建系统发育树。将7个BAC克隆的重复单元分为SacI、KpnI、#11F02和#16E07亚家族。SacI和KpnI亚家族以前已报道过。多色荧光原位杂交（FISH）使用SacI或Kpn I亚家族探针导致不同的信号强度和染色体末端的位置，表明每个染色体末端具有satDNA亚家族的独特组成。例如，X染色体的p臂表现出与Y染色体的假常染色体区域（PAR）上的信号组成相似的信号组成，但与X染色体的q臂上的信号组成不同。在S.阔叶树基因组每个亚家族可用于FISH核型分析的探针。
The satellite DNA (satDNA) on the ends of chromosomes has been isolated and characterized in the dioecious plant Silene latifolia. BAC clones containing large numbers of repeat units of satDNA in a tandem array were isolated to examine the clustering of the repeat units. satDNA repeat units were purified from each isolated BAC clone and sequenced. To investigate pairwise similarities among the repeat units, a phylogenetic tree was constructed using the neighbor-joining algorithm. The repeat units derived from 7 BAC clones were grouped into SacI, KpnI, #11F02, and #16E07 subfamilies. The SacI and KpnI subfamilies have been reported previously. Multicolored fluorescence in situ hybridization (FISH) using SacI or KpnI subfamily probes resulted in different signal intensities and locations at the chromosomal ends, indicating that each chromosomal end has a unique composition of subfamilies of satDNA. For example, the p arm of the X chromosome exhibited signal composition similar to that on the pseudo autosomal region (PAR) of the Y chromosome, but not to that on the q arm of the X chromosome. The satDNA has not been completely homogenized in the S. latifolia genome. Each subfamily is available for a probe of FISH karyotyping.