Ripening-associated biochemical traits of Cantaloupe Charentais melons expressing an antisense ACC oxidase transgene
Ripening-associated biochemical traits of Cantaloupe Charentais melons expressing an antisense ACC oxidase transgene
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DOI：
10.21273/jashs.122.6.748
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发表时间：
1997-11-01
影响因子：
1.9
通讯作者：
Latche, A
中科院分区：
文献类型：
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作者：
Guis, M;Botondi, R;Latche, A
转基因哈密瓜(Cucumis Melo var，Cantalupensis Naud.)与野生型果实相比，转基因果实没有发生明显的果皮黄化和果肉软化，但用50亩L~(-1)外源乙烯处理可以完全逆转这些影响，果肉在跃变开始之前就开始着色，因此不受乙烯抑制的影响，两种类型的果实中可溶性固形物以相同的速度积累，直到授粉后38天野生型果实脱落。由于乙烯抑制的果实没有形成花梗离区，它们仍然附着在植株上，积累了更多的糖，主要是蔗糖。收获转基因果实后，体内乙烯含量略有增加，但与果肉软化有关。
Transgenic Cantaloupe Charentais melons (Cucumis melo var, cantalupensis Naud. 'Vedrantais') exhibiting strong inhibition of ethylene production were used as a model to discriminate between ethylene-regulated and ethylene-independent ripening pathways, Compared to wild-type fruit, transgenic fruit did not undergo significant yellowing of the rind and softening of the flesh, However, these effects were completely reversed by treating transgenic fruit with 50 mu L.L-1 exogenous ethylene, Pigmentation of the flesh occurred early before the onset of the climacteric and was thus unaffected by ethylene inhibition in transgenic fruit, Total soluble solids accumulated at the same rate in both types of fruit until 38 days after pollination when wild-type fruit abscissed, However, as ethylene-inhibited fruit failed to develop a peduncular abscission zone, they remained attached to the plant and accumulated higher amounts of sugars, mainly sucrose, Harvesting transgenic fruit resulted in a small hut significant increase of internal ethylene associated with softening of the flesh.