Hierarchical Porous Protein Scaffold Templated from High Internal Phase Emulsion Costabilized by Gelatin and Gelatin Nanoparticles.
Hierarchical Porous Protein Scaffold Templated from High Internal Phase Emulsion Costabilized by Gelatin and Gelatin Nanoparticles.
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DOI：
10.1021/acs.langmuir.7b04047
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发表时间：
2018-04
期刊：
影响因子：
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通讯作者：
Huan Tan;Zhao Tu;H. Jia;X. Gou;To Ngai
中科院分区：
文献类型：
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作者：
Huan Tan;Zhao Tu;H. Jia;X. Gou;To Ngai
近年来，利用聚-皮克林高内相乳液（poly-Pickering high internal phase emulsions，polyHIPES）技术制备的三维（3D）支架在生物医学应用中特别有吸引力。然而，到目前为止，研究最多的聚HIPE是源自合成聚合物的疏水复合材料。在这里，我们提出了一个分层的多孔蛋白质支架模板的水包油（O/W）HIPES共稳定的全天然材料，明胶和明胶纳米粒子的调查。首先通过两步去溶剂化法合成了均匀分散的明胶纳米粒子，然后将其与明胶一起用作乳化剂，制备了具有可调液滴尺寸分布和流变性的稳定HIPE。通过在连续相中交联HIPE与低至2.5wt%的聚合物形成整体支架，其适当地呈现出一般的高孔隙率，并且具有具有光滑孔壁和纹理结构的互连多孔形态。此外，该支架可降解，具有良好的生物相容性; L929细胞可以粘附在材料表面，并表现出密集的生长和良好的铺展形态。因此，这种分级多孔蛋白质支架可以作为提供增强的细胞粘附和功能的3D支架具有重要的应用。
Recently, three-dimensional (3D) scaffolds produced using poly-Pickering high internal phase emulsions (polyHIPEs) technology are particularly attractive in biomedical application. However, until now the most investigated polyHIPEs are hydrophobic composites originating from synthetic polymers. Here we present an investigation of a hierarchical porous protein scaffold templated from oil-in-water (O/W) HIPEs costabilized by fully natural materials, gelatin, and gelatin nanoparticles. Fairly monodispersed gelatin nanoparticles were first synthesized through a two-step desolvation method, and then they were used as emulsifiers together with gelatin to fabricate stable HIPEs with adjustable droplet size distribution and rheology. Monolithic scaffolds were formed by cross-linking the HIPEs with polymers as low as 2.5 wt % in the continuous phase, which appropriately presented a general high porosity and had an interconnected porous morphology with smooth pore walls and textured structures. Furthermore, the scaffolds were degradable and showed reasonably good biocompatibility; L929 cells could adhere to the surface of the materials and exhibited intensive growth and well-spread morphology. This hierarchical porous protein scaffold could, therefore, have important application as a 3D scaffold that offers enhanced cell adhesion and functionality.