Consortia modulation of the stress response: proteomic analysis of single strain versus mixed culture
Consortia modulation of the stress response: proteomic analysis of single strain versus mixed culture
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DOI：
10.1111/j.1462-2920.2010.02217.x
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发表时间：
2010-09-01
影响因子：
5.1
通讯作者：
Martins dos Santos, Vitor A. P.
中科院分区：
文献类型：
--
作者：
Bobadilla Fazzini, Roberto A.;Preto, Maria J.;Martins dos Santos, Vitor A. P.
自然界微生物群落的高度复杂性是限制这些重要生物系统研究的主要缺点。在这项研究中，纯培养物的假单胞菌reinekei。菌株MT 1和稳定的社区文化组成的MT 1加上添加木糖氧化无色杆菌菌株MT3（在一个稳定的状态比例9：1）之间的比较被用来作为一个模型系统，研究细菌的相互作用，发生在同时化学和氧化应激。两者都是通过富集4-氯水杨酸（4CS）从污染沉积物中分离的真实的群落的成员。在蛋白质组、代谢谱和种群动态水平上进行动态分析。在暴露于4CS和高浓度的毒性中间体（4-氯儿茶酚和原白头翁素），包括来自几个官能团的蛋白质，特别是芳香族降解途径和外膜蛋白的酶下，评估差异蛋白质表达。值得注意的是，4CS的添加产生了强烈的氧化应激反应，在纯菌株MT 1培养的烷基过氧化氢还原酶领导的，而社区表现出增强的中央代谢反应，其中A.木糖氧化物MT3有助于防止有毒中间体积累。在4CS引起的化学胁迫和A. xylosoxidans MT3，强调了主要外膜蛋白OprF的表达，与4CC浓度分布及其潜在的解毒作用密切相关。
P>The high complexity of naturally occurring microbial communities is the major drawback limiting the study of these important biological systems. In this study, a comparison between pure cultures of Pseudomonas reinekei sp. strain MT1 and stable community cultures composed of MT1 plus the addition of Achromobacter xylosoxidans strain MT3 (in a steady-state proportion 9:1) was used as a model system to study bacterial interactions that take place under simultaneous chemical and oxidative stress. Both are members of a real community isolated from a polluted sediment by enrichment in 4-chlorosalicylate (4CS). The analysis of dynamic states was carried out at the proteome, metabolic profile and population dynamic level. Differential protein expression was evaluated under exposure to 4CS and high concentrations of toxic intermediates (4-chlorocatechol and protoanemonin), including proteins from several functional groups and particularly enzymes of aromatic degradation pathways and outer membrane proteins. Remarkably, 4CS addition generated a strong oxidative stress response in pure strain MT1 culture led by alkyl hydroperoxide reductase, while the community showed an enhanced central metabolism response, where A. xylosoxidans MT3 helped to prevent toxic intermediate accumulation. A significant change in the outer membrane composition of P. reinekei MT1 was observed during the chemical stress caused by 4CS and in the presence of A. xylosoxidans MT3, highlighting the expression of the major outer membrane protein OprF, tightly correlated to 4CC concentration profile and its potential detoxification role.