Replacement of owl monkey centromere satellite by a newly evolved variant was a recent and rapid process
Replacement of owl monkey centromere satellite by a newly evolved variant was a recent and rapid process
复制标题
DOI：
10.1111/gtc.12898
复制
发表时间：
2021-10-06
期刊：
影响因子：
2.1
通讯作者：
Koga，Akihiko
中科院分区：
文献类型：
--
作者：
Nishihara，Hidenori;Stanyon，Roscoe;Koga，Akihiko
α卫星DNA是灵长类着丝粒的主要DNA成分。我们之前报道过，Azara鸮猴有两种α卫星DNA， OwlAlp1和OwlAlp2。OwlAlp2 （344 bp）与近缘种的α卫星DNA在整个长度上具有相似性。OwlAlp1 （185 bp）对应OwlAlp2部分。根据观察到的CENP‐A蛋白与OwlAlp1结合，我们提出OwlAlp1是一个相对较新的重复DNA，取代了OwlAlp2作为着丝粒卫星DNA。然而，这种着丝粒DNA替换的进化过程的详细情况在很大程度上仍然未知。本文对OwlAlp1和OwlAlp2序列进行了系统发育分析，并与其他灵长类动物的α卫星DNA序列进行了比较。研究发现：(1)OwlAlp1与其他物种的α卫星DNA的相似性高于OwlAlp2， (2) OwlAlp1具有单一起源，(3)OwlAlp1的序列变异小于OwlAlp2。我们得出结论，OwlAlp1在猫头鹰猴谱系中经历了最近的快速扩展。这种着丝粒DNA的替换可能是由异染色质重组促成的，异染色质重组与猫头鹰猴适应夜间生活方式有关。
Alpha satellite DNA is a major DNA component of primate centromeres. We previously reported that Azara's owl monkey has two types of alpha satellite DNA, OwlAlp1 and OwlAlp2. OwlAlp2 (344 bp) exhibits a sequence similarity throughout its entire length with alpha satellite DNA of closely related species. OwlAlp1 (185 bp) corresponds to the part of OwlAlp2. Based on the observation that the CENP‐A protein binds to OwlAlp1, we proposed that OwlAlp1 is a relatively new repetitive DNA that replaced OwlAlp2 as the centromeric satellite DNA. However, a detailed picture of the evolutionary process of this centromere DNA replacement remains largely unknown. Here, we performed a phylogenetic analysis of OwlAlp1 and OwlAlp2 sequences, and also compared our results to alpha satellite DNA sequences of other primate species. We found that: (i) OwlAlp1 exhibits a higher similarity to OwlAlp2 than to alpha satellite DNA of other species, (ii) OwlAlp1 has a single origin, and (iii) sequence variation is lower in OwlAlp1 than in OwlAlp2. We conclude that OwlAlp1 underwent a recent and rapid expansion in the owl monkey lineage. This centromere DNA replacement could have been facilitated by the heterochromatin reorganization that is associated with the adaptation of owl monkeys to a nocturnal lifestyle.