Therapeutic effects of muscovite to non-steroidal anti-inflammatory drugs-induced small intestinal disease
Therapeutic effects of muscovite to non-steroidal anti-inflammatory drugs-induced small intestinal disease
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DOI：
10.1016/j.ijpharm.2012.05.063
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发表时间：
2012-10-15
影响因子：
5.8
通讯作者：
Zhang Shuo
中科院分区：
文献类型：
--
作者：
Chao Guanqun;Zhang Shuo
目的/背景：非类固醇抗炎药(NSAIDs)对人和动物的小肠损伤作用已被证实。白云母是一种天然粘土，由铝和镁的不溶双硅酸盐组成。中国已开发并投放市场，用于治疗胃病。本研究旨在观察白云母灌胃给药对双氯芬酸所致大鼠肠道损伤的影响。方法：雄性SD大鼠灌胃白云母9d，末次灌胃给予抗炎剂量双氯芬酸。对小肠的解剖病变、绒毛高度、厚度和截面积进行定量分析。观察超微结构的变化。采用分光光度法检测血中内毒素水平。结果：白云母可减轻双氯芬酸对大鼠小肠的肉眼和组织学损伤。白云母组绒毛高度(139.8±/-13.2µm)高于模型组(86.6±/-17.1µm)(P<0.05)。其厚度和截面积指数均高于模型组。白云母组大鼠门静脉血中脂多糖水平(0.84+/-1.17EU/ml)低于模型组(4.52+/-0.98EU/ml)(P<；0.05)。白云母组EFG明显高于模型组(P<；0.05)。结论：白云母对双氯芬酸致清醒大鼠小肠损伤有保护作用。白云母能修复非甾体抗炎药所致的肠道损伤，至少部分是由于机械屏障功能的显著损伤和表皮生长因子的减少。(C)2012爱思唯尔B.V.保留所有权利。
Objective/Background: The ability of non-steroidal anti-inflammatory drugs (NSAIDs) to injure the small intestine has been well established in humans and animals. Muscovite is one kind of natural clay consisting of an insoluble double silicate of aluminum and magnesium. It has been developed and marketed in China for the treatment of gastric diseases. The present study was designed to examine the effects of intragastric treatment of muscovite on the intestinal damage induced by administration of diclofenac in rat.Methods: Male SD rats were treated with muscovite for 9 days, with concomitant treatment with anti-inflammatory doses of diclofenac on the final 5 days. The anatomical lesion, villous height, the thickness and the section area of small intestine were quantitatively analyzed. The change of ultrastructural organization was observed. Endotoxin level in blood was measured by photometry. Epidermal growth factor was observed by immunohistochemistry.Results: Muscovite decreased the macroscopic and histologic damage induced by diclofenac in the rat small intestine. In the muscovite group, villous height (139.8 +/- 13.2 mu m) was higher than which of the model group (86.6 +/- 17.1 mu m) (P < 0.05). The index of the thickness and the section area was higher than model group. LPS level in the portal blood of muscovite (0.84 +/- 1.17 EU/ml) was lower than model group (4.52 +/- 0.98 EU/ml) (P < 0.05). The EFG of muscovite group was higher significantly compared with the model group (P < 0.05).Conclusion: Muscovite can protect the small intestine from the damage induced by diclofenac in the conscious rat. Muscovite can repair NSAID-induced intestinal damage at least in part because of significant lesion in mechanical barrier function and reduction in epidermal growth factor. (c) 2012 Elsevier B.V. All rights reserved.