Glandular tissue from human pancreas and salivary gland yields similar stem cell populations
Glandular tissue from human pancreas and salivary gland yields similar stem cell populations
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DOI：
10.1016/j.ejcb.2009.02.187
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发表时间：
2009-07-01
影响因子：
6.6
通讯作者：
von Briesen, Hagen
中科院分区：
文献类型：
--
作者：
Gorjup, Erwin;Danner, Sandra;von Briesen, Hagen
来源于胰腺组织的干细胞被很好地表征，并且表现出广泛的可塑性，因为它们可以分化超出谱系边界成许多细胞类型。本研究的目的是比较表征胰腺干细胞与胚胎前肠的其他衍生物，即唾液腺，类似的干细胞群的存在。采用逆转录聚合酶链反应、流式细胞术和免疫细胞化学染色检测干细胞标志物和谱系特异性标志物的表达。从唾液腺和胰腺分离的细胞在体外贴壁生长，并可分别维持高达55和46个群体倍增时间。来自两种组织的细胞显示出相当的表型。它们表达不同的胚胎干细胞和成体干细胞标志物，并具有自发分化为代表三个胚胎胚层的细胞的能力。此外，实现了腺干细胞向中胚层谱系的定向分化，从唾液腺干细胞产生成脂细胞、成骨细胞和成软骨细胞，以及从胰腺干细胞产生成骨细胞和成软骨细胞。在这里，我们比较了两个干细胞群体从不同的腺组织表现出相似的表型和类似的属性。在胚胎发育过程中，两个外分泌腺起源于前肠，这可能是这些有趣的同源性的解释。(C)2009年Elsevier GmbH。All rights reserved.
Stem cells derived from pancreatic tissue are well characterized and exhibit a broad plasticity as they can differentiate beyond lineage boundaries into many cell types. The aim of this study was the comparative characterization of pancreatic stem cells with one other derivate of the embryonic foregut, namely salivary glands, for the existence of similar stem cell populations. The expression of stem cell markers as well as lineage-specific markers was detected by reverse transcription polymerase chain reaction, flow cytometry and immuncytochemical staining. The isolated cells from salivary glands and pancreas grew adherently in vitro and could be maintained for up to 55 and 46 population doublings, respectively. Cells from both tissues showed a comparable phenotype. They expressed different embryonic and adult stem cell markers and had the ability to differentiate spontaneously into cells representing the three embryonic germ layers. Additionally, the directed differentiation of glandular stem cells into the mesodermal lineage was achieved, yielding adipogenic, osteogenic and chondrogenic cells from salivary gland stem cells as well as osteogenic and chondrogenic cells from pancreatic stem cells. Here, we compared two stem cell populations from different glandular tissues which showed similar phenotypes and analogous properties. During embryonic development the two exocrine glands originate from the foregut, which might be the explanation for these intriguing resemblances. (C) 2009 Elsevier GmbH. All rights reserved.