A Novel Solid Medium for Culturing Mycobacterium tuberculosis Isolates from Clinical Specimens
A Novel Solid Medium for Culturing Mycobacterium tuberculosis Isolates from Clinical Specimens
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DOI：
10.1128/jcm.01149-15
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发表时间：
2015-08-01
影响因子：
9.4
通讯作者：
Drancourt, M.
中科院分区：
文献类型：
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作者：
Asmar, S.;Chatellier, S.;Drancourt, M.
结核病的实验室诊断通常依赖于对致病结核分支杆菌的培养分离。我们开发并评估了MOD 9的性能，MOD 9是MOD 4固体培养基的一种新的无血衍生物，我们以前曾报道过用于分枝杆菌的分离和培养。首先，用21 M. 10（5）、10（3）和10 CFU的结核分离株在5.7 +/-1.5天、7.6 +/-0.8天和10.8 +/-1.7天内产生菌落，而在MOD 9中为1.5 +/-0.4天、3.5 +/-0.6天和4.9 +/-1天（P < 0.05，Student ′ s t检验）。此外，M.在用0.7%洗必泰消毒的250个临床标本的两次接种后，在MOD 9和Lowenstein-Jensen培养基上测量结核病。MOD 9的污染率为1.6%（4/250），而Lowenstein-Jensen培养基的污染率为4.4%（11/250）（P = 0.11，Fisher精确检验）。洗必泰-MOD 9产生38/250（15.2%）分离株，而洗必泰-LJ产生32/250（12.8%）分离株（P = 0.5195，Fisher精确检验）。加起来，8个M。结核分离株仅从洗必泰-MOD 9培养，两株M.结核分离株仅从洗必泰-LJ培养。洗必泰-MOD 9的检测时间为9.8 +/- 3.9（范围，5至18）天，而洗必泰-LJ的检测时间为17.4 +/- 5.9（范围，10至35）天（P < 0.05，学生t检验）。这些数据表明，在洗必泰去污后，MOD 9培养基优于标准LJ培养基用于M的回收。结核
The laboratory diagnosis of tuberculosis usually relies on culture-based isolation of the causative Mycobacterium tuberculosis bacteria. We developed and evaluated the performance of MOD9, a new blood-free derivative of the MOD4 solid medium on which we previously reported for the isolation and culture of mycobacteria. First, inoculation of Lowenstein-Jensen medium with 21 M. tuberculosis isolates at 10(5), 10(3), and 10 CFU yielded colonies in 5.7 +/- 1.5 days, 7.6 +/- 0.8 days, and 10.8 +/- 1.7 days versus 1.5 +/- 0.4 days, 3.5 +/- 0.6 days, and 4.9 +/- 1 days in MOD9 (P < 0.05, Student's t test). Further, the time to detectable growth of M. tuberculosis was measured on MOD9 and Lowenstein-Jensen media after duplicate inoculation of 250 clinical specimens decontaminated with 0.7% chlorhexidine. The contamination rate was 1.6% (4/250) on MOD9 versus 4.4% (11/250) on Lowenstein-Jensen medium (P = 0.11, Fisher's exact test). Chlorhexidine-MOD9 yielded 38/250 (15.2%) isolates versus 32/250 (12.8%) isolates for the chlorhexidine-LJ (P = 0.5195, Fisher's exact test). All together, eight M. tuberculosis isolates were cultured solely from chlorhexidine-MOD9, and two M. tuberculosis isolates were cultured solely from chlorhexidine-LJ. The time to detection was 9.8 +/- 3.9 (range, 5 to 18) days for chlorhexidine-MOD9 versus 17.4 +/- 5.9 (range, 10 to 35) days for chlorhexidine-LJ (P < 0.05, Student's t test). These data indicate the superiority of the MOD9 medium over the standard LJ medium following chlorhexidine decontamination for the recovery of M. tuberculosis.