Microcosm Biofilm Formation on Titanium Surfaces

Microcosm Biofilm Formation on Titanium Surfaces
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DOI:
10.1590/1516-1439.346814
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发表时间:
2015-08-01
期刊:
Materials Research
影响因子:
--
通讯作者:
Santos, Luciana Ruschel dos
Santos, Luciana Ruschel dos
中科院分区:
其他
文献类型:
--
作者:
Dal’Agnol, Claudio Zeferino;Stefenon, Letícia;Santos, Luciana Ruschel dos

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与种植体周围炎相关的危险因素与种植体周围的生物膜组成和细菌粘附能力有关。本研究使用微观世界技术和扫描电子显微镜(SEM)评价了12、24、48和168小时后4级商业纯钛(CP Ti)不同表面上的生物膜形成。检查以下表面条件:S(对照)-光滑和平整; B-用氧化铝喷砂; E-用硝酸蚀刻;和BE-J和A处理的组合。在前12小时,在对照(S)和B表面之间发现生物膜形成(CFU/mg)的统计学差异,其与最低(R-a = 0.21 μ m)和最高(R-a = 0.62 μ m)平均粗糙度值相关。在168小时时,所有表面均显示出相似的生物膜形成。然而,无论表面处理如何,微生物生长都发生在所有表面上。
Risk factors associated with peri-implantitis are related to the biofilm composition around the implant and the ability of bacterial adhesion. This study evaluated the biofilm formation on different surfaces of commercially pure titanium (CP Ti) grade 4 after 12, 24, 48 and 168 hours using the microcosm technique and scanning electron microscopy (SEM). The following surface conditions were examined: S (control)- smooth and plain; B- sand-blasted with aluminum oxide; E- etched using nitric acid; and BE-the combination of J and A treatments. Statistical differences on biofilm formation (CFU/mg) were found between the control (S) and B surfaces at the first 12 hours, which are related to the lowest (R-a = 0.21 mu m) and highest (R-a = 0.62 mu m) mean roughness values. At 168-hour all surfaces showed similar biofilm formation. Yet, microbial growth occurred on all surfaces, regardless of the surface treatment.