Highly amplified electrochemiluminescence of peroxydisulfate using bienzyme functionalized palladium nanoparticles as labels for ultrasensitive immunoassay.
Highly amplified electrochemiluminescence of peroxydisulfate using bienzyme functionalized palladium nanoparticles as labels for ultrasensitive immunoassay.
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DOI：
10.1016/j.bios.2012.06.004
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发表时间：
2013-01
影响因子：
12.6
通讯作者：
Huan Niu;R. Yuan;Y. Chai;Li Mao;Huijing Liu;Yaling Cao
中科院分区：
文献类型：
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作者：
Huan Niu;R. Yuan;Y. Chai;Li Mao;Huijing Liu;Yaling Cao
首次提出了一种基于过二硫酸盐电化学发光（ECL）的免疫传感器，该传感器以钯纳米粒子（PdNPs）为催化剂，通过偶联两种酶的协同作用原位生成共反应物。PdNPs是先前合成的，其成功地附着到功能性碳纳米管（FCNT）上，以结合二抗和双酶（辣根过氧化物酶和葡萄糖氧化酶）。然后通过夹心免疫反应将制备的生物缀合物引入电极。相应地，对于GOD催化葡萄糖产生H2 O2，其随后被HRP还原以原位产生O2，然后PdNPs作为过二硫酸盐/O2的ECL反应的催化剂，获得显著放大的ECL信号。该传感器用于甲胎蛋白（AFP）的检测，线性范围为1×10−5- 100 ngmL −1，检测限为3.3fgmL−1（S/N=3）。这种新的信号放大策略制备的ECL免疫传感器，可以很容易地实现，并在超灵敏的生物检测的潜在应用。
An immunosensor based on the electrochemiluminescence (ECL) of peroxydisulfate was firstly proposed by coupling the cooperation of two enzymes to in situ generate coreactant with palladium nanoparticles (PdNPs) as catalyst for the ECL reaction. PdNPs were previously synthesized, which successfully attached to functional carbon nanotubes (FCNTs), to bind the secondary antibody and bienzyme (horseradish peroxidase and glucose oxidase). Then the prepared bioconjugates were introduced to the electrode via sandwich immunoreactions. Accordingly, a dramatically amplified ECL signal was obtained for that GOD catalyzed glucose to produce H2O2which was subsequently reduced by HRP to in situ generate O2, then PdNPs as catalyst for the ECL reaction of peroxydisulfate/O2. The present immunosensor was used to detect α-1-fetoprotein (AFP) and showed a wide linear range of 1×10−5–100ngmL−1, with a low detection limit of 3.3fgmL−1(S/N=3). This new signal amplification strategy for preparation of the ECL immunosensor could be easily realized and has a potential application in ultrasensitive bioassays.