Ultrastructural, cyto- and biochemical observations during turnover of plasma membrane in duck salt gland.
Ultrastructural, cyto- and biochemical observations during turnover of plasma membrane in duck salt gland.
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鸭盐腺质膜周转过程中的超微结构、细胞和生化观察。
DOI：
10.1007/bf00222639
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发表时间：
1978
影响因子：
3.6
通讯作者：
Allen Er
中科院分区：
文献类型：
--
作者：
Hossler Fe;Sarras Mp;Allen Er
本文以雏鸭盐腺为模型系统，研究了盐腺细胞膜的更新机制。用ATP水解和~ 3 H-哇巴因结合法测定盐胁迫下雏鸭盐腺Na，K-ATP酶活性，结果表明，盐胁迫下雏鸭盐腺Na，K-ATP酶活性在7天内下降到非胁迫水平。电子显微照片显示，这是伴随着减少质膜内折的基础和侧边缘的腺体分泌细胞。同时，这些细胞中的丝状物质增加，酸性磷酸酶和肽酶活性升高。细胞化学显示酸性磷酸酶活性主要与分泌细胞的基底或基底外侧区有关。这些观察结果可能表明，质膜成分的去除是通过分泌细胞内的内化和消化完成的。
The mechanism of plasma membrane turnover was investigated using the duckling salt gland as a model system. Feeding fresh water to saltstressed ducklings results in a decrease in the Na, K-ATPase in salt gland to nonstressed levels in about 7 days, as measured by ATP hydrolysis and 3H-ouabain binding. Electron micrographs reveal that this is accompanied by a decrease in plasma membrane infoldings on the basal and lateral borders of gland secretory cells. Simultaneously there is an increase in filamentous material and a rise in acid phosphatase and peptidase activities in these cells. Cytochemistry shows that the acid phosphatase activity is mostly associated with the basal or basolateral regions of secretory cells. These observations could indicate that the removal of plasma membrane components is accomplished by internalization and digestion within the secretory cells.